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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 5621 - 5640 of 6562 results

  1. pYPQAT41

    Plasmid
    #223413
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants select.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223413-plasmid-map-sequence-id-452335

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  2. pYPQAT14

    Plasmid
    #223386
    Purpose
    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and sgRNA was by AtU3; BASTA for plant selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223386-plasmid-map-sequence-id-454178

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  16. Protein2PAM_NmeN4G.4

    Plasmid
    #250227
    Purpose
    Express Protein2PAM_NmeN4G.4 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4G.4
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 D957G/V979I/Q981A/L990V/I1010T/G1026S/N1…
    Promoter
    CMV
    Available since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250227-plasmid-map-sequence-id-502221

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  17. Protein2PAM_NmeN4CNNT.2

    Plasmid
    #250223
    Purpose
    Express Protein2PAM_NmeN4CNNT.2 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4CNNT.2
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 I1010K/F1017L/A1021I/C1023F/H1024D/I1034…
    Promoter
    CMV
    Available since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250223-plasmid-map-sequence-id-501763

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  18. pGGG-TaBSS2-tdTom

    Plasmid
    #242018
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic tdTomato driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    tdTomato
    Tags
    NLS
    Expression
    Plant
    Promoter
    BSS2
    Available since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    242018-plasmid-map-sequence-id-492286

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  19. pAAV-MP-84-spCas9-U6-gRNA-tlock

    Plasmid
    #206936
    Purpose
    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.
    Depositor
    Markus Grompe
    Insert
    spCas9
    Use
    AAV
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    MP-84
    Available since
    Sept. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    206936-plasmid-map-sequence-id-407723

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Showing: 5621 - 5640 of 6562 results