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pUC-TALO8

Plasmid

#83409

Purpose

TRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeast

Depositor

Toshi Tsukiyama

Publication

Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

Insert

TRP-ARS-8xLacO

Expression

Yeast

Mutation

Please see depositor comments below

Promoter

TRP1

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pME-nlsGFP-P2A

Plasmid

#80808

Purpose

nuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2A

Depositor

Philip Washbourne

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

minimal cytomegalovirus immediate early enhancer/promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSLeu2d-BISopt

Plasmid

#35524

Depositor

Taek Soon Lee

Publication

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

Bisabolene Synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

P GAL 1

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pE1B-C-F/Sox10-MCS7

Plasmid

#20246

Depositor

William Pavan

Publication

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS7

Expression

Mammalian

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pET28a-HA-CPDSalI

Plasmid

#38254

Depositor

Matthew Bogyo, Aimee Shen

Publication

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis, HA, and Vibrio cholerae MARTX toxin cystei…

Expression

Bacterial

Promoter

T7

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-HTB-hphMX4

Plasmid

#26876

Depositor

Peter Kaiser

Publication

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HTB tag

Use

Pcr tagging yeast

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HS-H4-GFP

Plasmid

#8699

Depositor

Kami Ahmad

Publication

Ahmad et al Mol Cell 2002 Jun;9(6):1191-200.

Insert

H4 (His4:CG31611 Fly)

Tags

GFP

Expression

Insect

Available since

June 30, 2005

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgATG7

Plasmid

#211534

Purpose

Deletes ATG7

Depositor

Richard Possemato

Publication

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgATG7

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGL0_1 [RK2 ORI]

Plasmid

#198918

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RK2 ORI

Use

Synthetic Biology

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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V518 pLPS-3'dMyc - Acceptor

Plasmid

#11714

Depositor

Tony Pawson

Publication

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

2xMyc

Expression

Mammalian

Available since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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V518 pLPS-3'dMyc - Acceptor

Plasmid

#11714

Depositor

Tony Pawson

Publication

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

2xMyc

Expression

Mammalian

Available since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pZac2.1 U6-SaDMDR7-U6-SaDMDL2

Plasmid

#78603

Purpose

AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Publication

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SaDMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only
ZO-1 shRNA 3253

Plasmid

#37206

Depositor

James Anderson, Alan Fanning

Publication

Van Itallie et al Mol Biol Cell. 2009 Sep;20(17):3930-40.

Insert

dog ZO-1 shRNA (TJP1 canine)

Use

RNAi

Expression

Mammalian

Available since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pYAL_HLA-A2_MART1

Plasmid

#171177

Purpose

Displays MART-1 peptide in HLA-A*02 as a single-chain trimer

Depositor

Michael Birnbaum

Publication

Huisman et al Methods Mol Biol. 2022;2491:263-291. doi

Insert

MART1_HLA-A2*01 single-chain trimer fusion

Expression

Bacterial and Yeast

Promoter

GAL1

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-attL1-pegRNA-attR5

Plasmid

#213053

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5

Depositor

Bing Yang

Publication

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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U6-AsCas12f-full-length-sgRNA

Plasmid

#204638

Purpose

Expression of full-length AsCas12f sgRNA in mammalian cells

Depositor

Weixin Tang

Publication

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

full-length AsCas12f sgRNA

Expression

Mammalian

Promoter

U6

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pQCascade-IS1

Plasmid

#140624

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS1. The crRNA-IS1 targets IS1 loci in Escherchia coli.

Depositor

Sheng Yang

Publication

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1

Use

CRISPR; Transposon

Expression

Bacterial

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSW068

Plasmid

#140036

Purpose

KO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

Depositor

Peter Nixon

Publication

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Truncated thioesterase gene

Expression

Bacterial

Promoter

cLac143

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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Bongwoori-Pos6

Plasmid

#101655

Purpose

Bongwoori with the CC1-Pos6 linker length and composition.(* super ecliptic pHluorin A227D)

Depositor

Bradley Baker

Publication

Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Bongwoori-Pos6

Expression

Mammalian

Available since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPBC-G6s

Plasmid

#62812

Purpose

pPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.

Depositor

Karen Wilcox

Publication

Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

Insert

ITR-CAG-GCaMP6s-ITR

Expression

Bacterial and Mammalian

Promoter

CAG

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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