We narrowed to 63,044 results for: cloning
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Plasmid#136899PurposeLentivirus for base editing activatable GFP expression in mammalian cellsDepositorInsertGFPAdGO2
UseLentiviralTags2xNLSMutationTGG>TAGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
M3D395A-M14-dCas9
Plasmid#134780PurposeExpression of fusion protein M3D395A-M14-dCas9 in Mammalian cellsDepositorInsertMETTL3/METTL14 methyltransferase domain and inactive dCas9
ExpressionMammalianMutationD395APromoterCMVAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST mfap4:turquoise2
Plasmid#135218PurposeCyan tagged macrophagesDepositorInsertmfap4 promoter and mTurquoise2
UseZebrafish expressionAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCP-tRNA
Plasmid#133812PurposeCRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosisDepositorInsertcassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
UseCRISPRAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Target-AID (pRZ762)
Plasmid#131300PurposeCAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.DepositorInsertNLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
ExpressionMammalianPromoterCAGAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Yeast GPCR-sensor Toolkit
Plasmid kit#1000000157PurposeThis toolkit includes plasmids for constructing highly-tunable GPCR-based biosensors in yeast.DepositorApplicationCloning and Synthetic Biology, Gene Expression and LabelingVector typeYeast ExpressionCloning typeGolden Gate (MoClo)Available sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
TBP6.7 non-targeting gRNA lambda phage
Plasmid#132547Purposeexpresses gRNA for TBP-based CIRTSDepositorInsertgRNA TBP-based CIRTS
ExpressionMammalianPromoterhU6 promoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-Turbo-NES-YFP-STOP-L2
Plasmid#127358Purposegateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choiceDepositorInsertTurboID (BirA mutant)
UseGateway CloningTagsGS linker, NES, V5, and YFPMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-KIF5B*-strep
Plasmid#120170PurposeExpresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidinDepositorInsertkinesin family member 5B (Kif5b Mouse)
TagsHA and StreptavidinExpressionMammalianMutationTruncated KIF5B: 1-555PromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-SomArchon
Plasmid#126942PurposeExpression of SomArchon under CaMKII promoterDepositorInsertSomArchon
UseAAVTagsGFPExpressionMammalianPromoterCaMKIIAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTOX1
Plasmid#127446PurposeEncodes YhaV toxin. CAM-RDepositorInsertyhaV toxin
ExpressionBacterialPromoterpRhaAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-TetON-dual-SunTag-Hygro
Plasmid#121119PurposeEndogenous gene activation with the SunTag CRISPR activation systemDepositorInsertdCas9-GCN4x10_scFv-VP64
UseCRISPRExpressionMammalianPromoterTet-ONAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas9-PCV
Plasmid#123644PurposeExpresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coliDepositorAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_POU2F3_CR
Plasmid#122175PurposeLentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance geneDepositorInsertPOU2F3 (POU2F3 Human)
UseLentiviralExpressionMammalianMutation3 silent mutations for gRNA resistance (882g-c, 8…PromoterEFSAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAR17_StrepI_hFASh_H8_pET22b
Plasmid#122846PurposeExpresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInserthuman FASN/ human fatty acid synthase (FASN Human)
TagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-IGK-dAPEX2-KDEL
Plasmid#117182PurposePlasmid name in publication: pAAV-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertIGK-dAPEX2-KDEL
UseAAVExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAR357_SFP_pCDF-1b
Plasmid#122851PurposeCo-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.DepositorInsertSfp
ExpressionBacterialPromoterT7 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ESR1-Y537S
Plasmid#116374PurposeLentiviral expression of ESR1 Y537SDepositorAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1
Plasmid#122083PurposeExpress M.EcoGII fused to LaminB1 with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII-V5-LaminB1
UseRetroviralTagsV5 tag on Nter of LaminB1ExpressionBacterial and MammalianAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MiniCoopR 2xU6:gRNA, mitfa:Cas9
Plasmid#118844PurposeTargets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorTypeEmpty backboneUseCRISPR; Tol2Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by