We narrowed to 14,812 results for: Coli
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Plasmid#37571DepositorAvailable SinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits only
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pHL1559
Plasmid#37570DepositorAvailable SinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHL1324
Plasmid#37638DepositorInsertsGFP
TetR
CsrA
UseSynthetic BiologyExpressionBacterialPromoterpCon and pConNoHindAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
HL 3076 (=HL 716 + pHL 1069)
Plasmid#30036DepositorInsertpCon:MicC, pLlacO-1:sodB::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialAvailable SinceSept. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 4179 (=HL 865 + pHL 1381)
Plasmid#30098DepositorInsertpLlacO-1:hfq, pLtetO-1:rpoS::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialAvailable SinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 4202 (=HL 716 + pHL 1389)
Plasmid#30100DepositorInsertpLtetO-1:DsrA, pLlacO-1:rpoS::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialAvailable SinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 4178 (=HL 862 + pHL 1360)
Plasmid#30097DepositorInsertpLlacO-1:hfq, pLtetO-1:ompC::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialAvailable SinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSL5 SMR1713
Plasmid#28028DepositorInsertMutL (mutL E. coli)
ExpressionBacterialAvailable SinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLII282Pglu-4m
Plasmid#13564DepositorInsertFLIPglu F16A T282S-ECFP-N283R
TagsCitrineExpressionBacterialMutationF16A, ECFP insert in binding proteinAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pESdelta 799
Plasmid#13042DepositorInsertMutS
TagsHis6ExpressionBacterialMutationC-terminal deletion from aa 800 through 853.Available SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pOP1
Plasmid#112595PurposeUsed for expression of protein in E. coli as non-fusionsDepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEcCas
Plasmid#73227PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRNADepositorInsertsCas9
sgRNA
UseCRISPR; Red recombinaseExpressionBacterialPromoternative promoterAvailable SinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_1
Plasmid#78198PurposeExpression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A197C)
ExpressionBacterialMutationA197C (please see depositors comment below)PromoterT7Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas
Plasmid#62225PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRDepositorHas ServiceCloning Grade DNAInsertcas9
UseCRISPR; Lambda red recombinaseExpressionBacterialPromoternative cas9 promoterAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_2
Plasmid#78199PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,A197C)
ExpressionBacterialMutationA17C; A197C (please see depositor comment below)PromoterT7Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Alive
Plasmid#20860PurposeAlive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.DepositorInsertStreptavidin-Alive
TagsHisExpressionBacterialMutationWt geneAvailable SinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Dead
Plasmid#20859PurposeDead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).DepositorInsertStreptavidin-Dead
ExpressionBacterialMutationTriple mutant of wt Streptavidin N23A,S27D,S45AAvailable SinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCKmatBC
Plasmid#138587PurposematBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, catDepositorInsertbicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
UseSynthetic BiologyExpressionBacterialMutationmatB contains native GUG start codonPromoterPlac promoterAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGM1190
Plasmid#69994PurposeOriT containing mobilizable vector for thiostrepton induced gene expression in StreptomycesDepositorInsertsaac
tsr
rep
sso
TipA promoter
dso
ExpressionBacterialAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
P3U2C7T2-v1-1 (Pl-lacO1-deGFP) (134p)
Plasmid#70210Purpose1 Part of 4-part switchDepositorInsertdeGFP
Available SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only