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MiCy-pBAD

Plasmid

#54565

Purpose

Localization: Protein Expression Vector , Excitation: 472, Emission: 495

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Tags

6xHis and MiCy

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pHT-trxA.ads

Plasmid

#47383

Purpose

Expresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteria

Depositor

Heng-Phon Too

Article

Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

Insert

ads

Tags

6xHis and trxA

Expression

Bacterial

Promoter

Pgrac

Available Since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-GATA2

Plasmid

#1287

Depositor

Gokhan Hotamisligil

Article

Tong et al Science 2000 Oct 6;290(5489):134-8.

Insert

GATA2 (GATA2 Human)

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pORF1-2

Plasmid

#206024

Purpose

An expression vector for mouse LINE1 ORF1-2

Depositor

Masamichi Kamihira

Article

Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

Insert

ORF1-2 derived from mouse LINE1

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-NT

Plasmid

#164857

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-NT

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBabePuro-GATA3

Plasmid

#1286

Depositor

Gokhan Hotamisligil

Article

Tong et al Science 2000 Oct 6;290(5489):134-8.

Insert

GATA3 (Gata3 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pET21a - TR

Plasmid

#89621

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Thioredoxin

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pOO2-GW

Plasmid

#135097

Purpose

Gateway vector for expression of proteins in Xenopus oocytes

Depositor

Wolf Frommer

Article

Chen et al Nature. 2010 Nov 25;468(7323):527-32. do

Type

Empty backbone

Use

Bacterial expression vector for generating sp6 tr…

Promoter

SP6

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LV-TRE-p65 mouse MyoD-T2A-dsRedExpress2

Plasmid

#60627

Purpose

Expresses p65 mouse MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

p65 mouse MyoD

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgUpf1

Plasmid

#161810

Purpose

guide RNA for Upf1

Depositor

Yi Zhang

Article

Fu et al Sci Adv. 2020 May 29;6(22):eaay5181. doi

Insert

sgRNA targeting mouse Upf1 (Upf1 Synthetic, Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSP6-EBNA(2A+DBD)

Plasmid

#98749

Purpose

In vitro transcription of modified EBNA1 mRNA

Depositor

Sara Howden, Jim Vadolas

Article

Howden et al Hum Gene Ther. 2006 Aug;17(8):833-44.

Insert

Modified EBNA1

Use

In vitro transcription

Promoter

SP6

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pFU-AgaIVA-VG

Plasmid

#24145

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

Insert

Venus-AgatoxinIVA-FLAG-GPI anchor

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pUAS-syb:spGFP1–10

Plasmid

#73698

Purpose

Neuronal-synaptobrevin (syb):spGFP1–10 chimera for synaptic GRASP

Depositor

Marco Gallio

Article

Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

Insert

Neuronal-synaptobrevin:spGFP1–10 chimera

Use

Drosophila

Expression

Insect

Available Since

March 22, 2016

Availability

Academic Institutions and Nonprofits only

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pHelper_AraC/pBad-Tnpase (camR)

Plasmid

#213909

Purpose

Arabinose-inducible himar1C9 transposase expression - cam resistance

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

AraC/pBad ATc-inducible himar1C9 transposase

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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UAS:TVA-mCherry

Plasmid

#187823

Purpose

Expression of a fusion of the transmembrane protein TVA and the red fluorescent protein mCherry under UAS control.

Depositor

Rainer Friedrich

Article

Satou et al Elife. 2022 Jul 22;11. pii: 77153. doi:

Insert

TVA-mCherry

Expression

Bacterial

Promoter

5xUAS

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)

Plasmid

#92400

Purpose

Constitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and membrane tag

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pET28 Set9 wt

Plasmid

#24082

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Tags

FLAG and His

Expression

Bacterial

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA Set9 mutant

Plasmid

#24085

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

H297A

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA-GATA3

Plasmid

#1332

Depositor

Gokhan Hotamisligil

Article

Tong et al Science 2000 Oct 6;290(5489):134-8.

Insert

GATA3 (Gata3 Mouse)

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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FepA

Plasmid

#26039

Depositor

Johann Deisenhofer

Article

Buchanan et al Nat Struct Biol. 1999 Jan . 6(1):56-63.

Insert

FepA (fepA E Coli)

Expression

Bacterial

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only