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ABE-RA4.5

Plasmid

#208310

Purpose

Expresses ABE-RA4.5 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.5-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.6

Plasmid

#208311

Purpose

Expresses ABE-RA4.6 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.6-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.0

Plasmid

#208312

Purpose

Expresses ABE-RA5.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.0-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.1

Plasmid

#208313

Purpose

Expresses ABE-RA5.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.1-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.3

Plasmid

#208314

Purpose

Expresses ABE-RA5.3 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.3-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.4

Plasmid

#208315

Purpose

Expresses ABE-RA5.4 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.4-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, R47K, P48A, R51L, I76Y, V82S, A106V, D108G,…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.6

Plasmid

#208317

Purpose

Expresses ABE-RA5.6 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.6-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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PB-CAG-4300-L1-G1397N

Plasmid

#210396

Purpose

To correct m.A4300G mutation on mtDNA

Depositor

Bin Shen

Article

Chen et al Commun Biol. 2023 Nov 3;6(1):1116. doi:

Insert

4300-L1-G1397N

Expression

Mammalian

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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PB-CAG-4300-H2-G1397C

Plasmid

#210395

Purpose

To correct m.A4300G mutation on mtDNA

Depositor

Bin Shen

Article

Chen et al Commun Biol. 2023 Nov 3;6(1):1116. doi:

Insert

4300-H2-G1397C

Expression

Mammalian

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB560-NADK2

Plasmid

#203760

Purpose

For Agrobacterium transformation. tagRFP fused Arabidopsis thaliana NAD KINASE2 (NADK2) under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

NAD KINASE2

Expression

Bacterial and Plant

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDGE1176

Plasmid

#202774

Purpose

CRISPR-KO in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:zCas9_tNbEU
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDGE1177

Plasmid

#202775

Purpose

CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:TREX2-zCas9_tNbEU
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-Cys-HA-Ubiquitin

Plasmid

#185356

Purpose

In vitro ubiquitination assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Ubiquitin

Tags

GST, HA(x2)

Expression

Mammalian

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA23-sgRNA vector-U6-sgTS2 (SpCas9)

Plasmid

#199454

Purpose

U6-driven sgRNA targeting TS2 sequence (GGAGCTTACTGAGACTCTTC)

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TS2 sgRNA

Use

CRISPR

Promoter

mouse U6

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCA28-pMa-PspCas13b crRNA-TS1

Plasmid

#199459

Purpose

U6-driven crRNA targeting TS1 sequence (CCTCCTCGGAGAGCATCGGTGC )

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TS1 crRNA

Use

CRISPR

Promoter

mouse U6

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-R60f-BFP

Plasmid

#186608

Purpose

Encodes human Reticulon 2 disease mutant R60f fused to BFP

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 mutant R60f (RTN2 Human)

Tags

BFP

Expression

Mammalian

Mutation

c.178insC (R60f)

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Rtn2b-mNeon-TurboID

Plasmid

#186612

Purpose

Third generation lentiviral vector expressing full-length Reticulon 2 fused to mNeonGreen and V5-TurboID

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-NTD1-271-mNeon

Plasmid

#186601

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 271 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 1 to 271 (RTN2 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-NTD1-135-mNeon

Plasmid

#186602

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 135 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 1 to 135 (RTN2 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-NTD1-60-mNeon

Plasmid

#186603

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 60 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 1 to 60 (RTN2 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Ppst93-E50 MCR-NG

Plasmid

#192778

Purpose

Expresses NG-tagged E50 MCR in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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Ppst93-ANME2cLM014 MCR-NG

Plasmid

#192777

Purpose

Expresses NG-tagged ANME2cLM014 MCR in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Ppst93-ANME2cE20 MCR-NG

Plasmid

#192775

Purpose

Expresses NG-tagged ANME2cE20 MCR in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Ppst93-MCRaeo-ND

Plasmid

#192771

Purpose

Expresses ND-tagged MCRaeo in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Ppst93-MCRaeo-NC

Plasmid

#192770

Purpose

Expresses NC-tagged MCRaeo in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Ppst93-MCRaeo-NG mcrBGA

Plasmid

#192769

Purpose

Expresses NG-tagged MCRaeoBGA in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Ppst93-MCRaeo-NG mcrBCGA

Plasmid

#192768

Purpose

Expresses NG-tagged MCRaeoBCGA in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Ppst93-MCRaeo-NG mcrBDGA

Plasmid

#192767

Purpose

Expresses NG-tagged MCRaeoBDGA in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Ppst93-MCRmar-NG

Plasmid

#192766

Purpose

Expresses NG-tagged MCRmar in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Ppst93-MCRaeo-CA

Plasmid

#192765

Purpose

Expresses CA-tagged MCRaeo in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge