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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,282 results for: LEA

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Showing: 5701 - 5720 of 12282 results

  2. pCW57.1 DOX off puro IKBKG

    Plasmid
    #241439
    Purpose
    Expresses DOX-suppressible IKBKG
    Depositor
    Richard Possemato
    Insert
    IKBKG (IKBKG Human)
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Partly codon optimized
    Available since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    241439-plasmid-map-sequence-id-492414

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  3. pcDNA3-mCherry-TSC2(AA)

    Plasmid
    #192442
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.
    Depositor
    Jin Zhang
    Publication
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-TSC2(S939A,T1439A) (TSC2 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192442-plasmid-map-sequence-id-379128

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  4. pcDNA3-ERKsub(TA)DD-Venus-NES

    Plasmid
    #84631
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted
    Depositor
    Jin Zhang
    Publication
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ERKsub(TA)DD-Venus-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Mutated target Thr residue to Ala.
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    84631-plasmid-map-sequence-id-175151

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  5. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid
    #169164
    Purpose
    Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells
    Depositor
    John Diffley
    Publication
    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464
    Insert
    nsp14/nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    6His-3xFlag (nsp10 C-terminus)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169164-plasmid-map-sequence-id-333982

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  6. pGBW-m4046878

    Plasmid
    #145743
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145743-plasmid-map-sequence-id-298347

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  10. pRSFDuet-1_VNp-LZ_VNp-LZ-hGH

    Plasmid
    #182412
    Purpose
    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine zipper-human growth hormone fusion
    Depositor
    Dan Mulvihill
    Publication
    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396
    Inserts
    VNp-LZ-hGH (GH1 Human)
    VNp-LZ
    Tags
    Vesicle Nucleating peptide (VNp)
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    182412-plasmid-map-sequence-id-360650

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  11. pDisplay-GRAB_OTmut

    Plasmid
    #185385
    Purpose
    Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cells
    Depositor
    Yulong Li
    Publication
    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based oxytocin control sensor GRAB_OTmut
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    185385-plasmid-map-sequence-id-365773

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  13. pLenti GW V5 Eco/Dam mse EMD

    Plasmid
    #182675
    Purpose
    Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Emerin. Used in DamID experiments to methylate DNA that interacts in the proximity of Emerin.
    Depositor
    Bas van Steensel
    Publication
    Amendola et al EMBO Rep. 2015 May;16(5):610-7. doi: 10.
    Insert
    Emerin (Emd Mouse)
    Use
    Lentiviral
    Tags
    E. Coli Dam and V5
    Expression
    Mammalian
    Promoter
    HSP
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182675-plasmid-map-sequence-id-358621

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Showing: 5701 - 5720 of 12282 results