We narrowed to 11,387 results for: mpl

Showing: 8581 - 8610 of 11387 results

Results per page:

Sort results by:

pFA-DAB1

Plasmid

#162691

Purpose

Expresses DabAB1 inorganic carbon transport complex

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

DabAB1 inorganic carbon transport complex

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD150 ZF43x12-Spaced-Far_EYFP

Plasmid

#138895

Purpose

EYFP reporter vector for the ZF43x12-Spaced-Far promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Spaced-Far

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x12-Spaced-Far

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD147 ZF43x8-Spaced-Far_EYFP

Plasmid

#138893

Purpose

EYFP reporter vector for the ZF43x8-Spaced-Far promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x8-Spaced-Far

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x8-Spaced-Far

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD145 ZF43x4-Spaced-Far_EYFP

Plasmid

#138891

Purpose

EYFP reporter vector for the ZF43x4-Spaced-Far promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x4-Spaced-Far

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x4-Spaced-Far

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD144 ZF43x2-Spaced-Far_EYFP

Plasmid

#138890

Purpose

EYFP reporter vector for the ZF43x2-Spaced-Far promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x2-Spaced-Far

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x2-Spaced-Far

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD140 ZF43x12-Spaced-Mid_EYFP

Plasmid

#138889

Purpose

EYFP reporter vector for the ZF43x12-Spaced-Mid promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Spaced-Mid

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x12-Spaced-Mid

Available Since

Jan. 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD137 ZF43x8-Spaced-Mid_EYFP

Plasmid

#138887

Purpose

EYFP reporter vector for the ZF43x8-Spaced-Mid promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x8-Spaced-Mid

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x8-Spaced-Mid

Available Since

Jan. 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD135 ZF43x4-Spaced-Mid_EYFP

Plasmid

#138885

Purpose

EYFP reporter vector for the ZF43x4-Spaced-Mid promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x4-Spaced-Mid

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x4-Spaced-Mid

Available Since

Jan. 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD134 ZF43x2-Spaced-Mid_EYFP

Plasmid

#138884

Purpose

EYFP reporter vector for the ZF43x2-Spaced-Mid promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x2-Spaced-Mid

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x2-Spaced-Mid

Available Since

Jan. 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-Orf6

Plasmid

#157726

Purpose

mammalian expression of untagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Orf6 (ORF6 SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA5-FRT-TO-Nsp15

Plasmid

#157720

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp15 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA5-FRT-TO-Nsp14

Plasmid

#157717

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp14 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Scarlet I-FRET 10-Cerulean3

Plasmid

#139374

Purpose

FRET Standard

Depositor

Richard Day

Article

Day et al PLoS One. 2021 Dec 23;16(12):e0261660. d

Insert

Cerulean3 N1

Expression

Mammalian

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCool-human TSC1 (939-992)

Plasmid

#132618

Purpose

expresses human TSC1 (939-992) in E. coli

Depositor

Wenqing Xu

Article

Qin et al J Biol Chem. 2016 Apr 15;291(16):8591-60

Insert

TSC1 (939-992)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti4 Blast RFP-PNRC1

Plasmid

#123297

Purpose

Mammalian expression of PNRC1 N-RFP

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Use

Lentiviral

Tags

TurboRFP

Expression

Mammalian

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
px330 Gatad2a sgRNA (for deletion)

Plasmid

#110814

Purpose

Expressed sgRNA for generating Gatad2a deletion (knockout ) in mouse cell lines

Depositor

Jacob Hanna

Article

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Expression

Mammalian

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(P46A)

Plasmid

#112721

Purpose

This mutation (P46A) marks the first residue within the evolved region of U1A.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P46A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P46A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(R47A)

Plasmid

#112722

Purpose

Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R47A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(R47K)

Plasmid

#112723

Purpose

Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47K)

Tags

6His-TEV

Expression

Bacterial

Mutation

R47K

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(Q48A)

Plasmid

#112724

Purpose

Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48A)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(Q48T)

Plasmid

#112725

Purpose

This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48T)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48T

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(T50A)

Plasmid

#112727

Purpose

Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(T50A)

Tags

6His-TEV

Expression

Bacterial

Mutation

T50A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(P51A)

Plasmid

#112728

Purpose

Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P51A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P51A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(R52A)

Plasmid

#112729

Purpose

52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R52A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R52A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(Q54A)

Plasmid

#112730

Purpose

Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q54A)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q54A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFMW-AthHEN1-CM

Plasmid

#104981

Purpose

Expression of FLAG Myc tagged catalytic dead A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Insert

Hen1 (HEN1 Mustard Weed)

Tags

FLAG Myc

Expression

Insect

Promoter

Actin5C

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMflCT-o4

Plasmid

#101313

Purpose

Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Inserts

oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
cat resistance gene

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-REDD1 85-232 (#427)

Plasmid

#99922

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

N-terminal deletion; encodes AA 85-232

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-REDD1 1-192 (#421)

Plasmid

#99925

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

C-terminal deletion; encodes AA 1-192

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-REDD1 1-209 (#430)

Plasmid

#99926

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

C-terminal deletion; encodes AA 1-209

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge