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ePPEmax*

Plasmid

#205244

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS

Use

CRISPR

Expression

Plant

Mutation

Detailed in manuscript

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-Dyskerin

Plasmid

#207577

Purpose

N-terminal mCherry-tag using SalI/EcoRI

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

generated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI

Tags

mCherry

Expression

Mammalian

Promoter

LTR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCAT007

Plasmid

#171634

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-TRAC
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pL-SFFV.Reporter.RFP657.PAC

Plasmid

#61395

Purpose

Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-Alu-Lin28

Plasmid

#92346

Purpose

Plasmid for expression of GFP gene with a Lin28 gene derived Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Lin28 gene derived Alu element containing 3'UTR (LIN28A Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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bdSUMO-E5TAT-GFP-HiBit

Plasmid

#170993

Purpose

For bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-E5TAT-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pH2B-Electra1

Plasmid

#179478

Purpose

Nuclear labeling with blue fluorescent protein Electra1 fused to H2B

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

H2B-Electra1

Tags

Electra1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIN-EF1a-mNeonGreen-P2A-Cas9

Plasmid

#189807

Purpose

Retroviral delivery of Cas9

Depositor

Ellen Rothenberg

Article

Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

Insert

Cas9

Use

Retroviral

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-146

Plasmid

#118562

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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ePPE-V223A

Plasmid

#205243

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

SV40_NLS-nCas9-NC-MLV-SV40_NLS

Use

CRISPR

Expression

Plant

Mutation

Detailed in manuscript

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEF Synaptophysin-GCamp6s

Plasmid

#188981

Purpose

Presynaptic fluorescent Ca2+ indicator

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Synaptophysin (Syp Synthetic, Rat)

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Sox2-t2A-mCherry

Plasmid

#127538

Purpose

Expresses human SOX2 and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

SOX2 (SOX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAsCpf1(TYCV)(BB) (pY211)

Plasmid

#89352

Purpose

Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K607R

Promoter

CBh

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTol2-nbt-Electra2-P2A-mScarlet

Plasmid

#196114

Purpose

zebrafish expression of nbt-Electra2-P2A-mScarlet

Depositor

Reinhard Köster

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

nbt-Electra2-P2A-mScarlet

Use

Zebrafish expression

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.MARK2-Halo

Plasmid

#224414

Purpose

Mammalian expression of MARK2

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

MARK2 (MARK2 Human)

Tags

Halo

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA-Tet2

Plasmid

#85743

Purpose

shRNA against mouse Tet2

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

shRNA Tet2

Use

AAV

Tags

EYFP

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCLC3sGFP

Plasmid

#52423

Purpose

mammalian expression of rat CLC3 short transcript fused to EGFP

Depositor

Steven Weinman

Article

Li et al Am J Physiol Cell Physiol. 2002 Jun;282(

Insert

CLC3 (Clcn3 Rat)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-PI4KB

Plasmid

#221759

Purpose

Stable expression of GFP-PI4KB in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PI4KB (PI4KB Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1 PIK3R1

Plasmid

#17743

Depositor

Bert Vogelstein

Article

Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

Insert

PIK3R1 (PIK3R1 Human)

Expression

Insect

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pMRE-Tn5-140

Plasmid

#118524

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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