We narrowed to 41,904 results for: tro

1. (564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9

   Plasmid

   #163025

   Purpose

   Expression of CRISPRi with gRNA against mouse Pcsk9

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9 (Pcsk9 S. aureus)
   

   Use

   AAV

   Tags

   KRAB, SV40 pA, and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Alb-AAT

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDisplay-GRAB_CRF1.0

   Plasmid

   #208655

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFC902

   Plasmid

   #106904

   Purpose

   The vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al Fungal Genet Biol. 2018 Jan 8. pii: S108

   Inserts

   cas9
   pyrG
   U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. BLBP-mCherry

   Plasmid

   #63721

   Purpose

   Expressing mCherry from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

   Depositor

   Bart De Strooper

   Article

   Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

   Insert

   BLBP (Fabp7 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1700 bp upstream of BLBP gene cloned in CAG-mCher…

   Promoter

   1700bp upstream of BLBP gene

   Available since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. H3S10ph biosensor

   Plasmid

   #120809

   Purpose

   FRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRET

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Threonine 3, Threonine 6, and Threonine 11 are mu…

   Promoter

   CMV

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDNR-SpCas9-Gem

   Plasmid

   #80424

   Purpose

   Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

   Depositor

   Melissa Little, Sara Howden

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Gem
   

   Use

   CRISPR

   Tags

   Geminin

   Expression

   Mutation

   codon optimized for human

   Promoter

   T7

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pD40-His/V5-c-MycT58A

   Plasmid

   #45598

   Depositor

   Rosalie Sears

   Article

   Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

   Insert

   c-Myc T58A (Myc Mouse)
   

   Use

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   T58A

   Promoter

   CMV

   Available since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pCS2plus-mCerulean 156-239-GGGS

   Plasmid

   #162616

   Purpose

   Allows for transcription of control mCerulean fluorophore half for injection into zebrafish embryos for BiFC assays

   Depositor

   Marco Morsch

   Article

   Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

   Insert

   mCerulean
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   156-239

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRF1R-GFP

   Plasmid

   #208912

   Purpose

   encodes the human corticotropin releasing factor 1 receptors, tagged with YFP

   Depositor

   Christian Gruber

   Article

   Fahradpour et al Front Pharmacol. 2017 Sep 25;8:616. doi:

   Insert

   CRHR1 (CRHR1 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-GFP-IMP2-2

    Plasmid

    #42175

    Depositor

    Alexandra Kiemer

    Article

    Kessler et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Insulin-like growth factor 2 mRNA binding protein 2-2 (IGF2BP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

11. AAV-TRE-NS1-dTomato

    Plasmid

    #175278

    Purpose

    AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    NS1; dTomato (POLY Yellow fever virus strain 17D)
    

    Use

    AAV

    Tags

    dTomato (from bidirectional promoter)

    Expression

    Mutation

    Promoter

    bidirectional TRE promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2plus-mVenus1-155-GGGS

    Plasmid

    #162610

    Purpose

    Allows for transcription of control mVenus fluorophore half for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    mVenus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains aa1-155 of mVenus

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEBTet-SNAP-Cep63

    Plasmid

    #136811

    Purpose

    Mammalian expression of the centrosomal protein Cep63 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep63 (CEP63 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA 3.1 mitoAURKA

    Plasmid

    #157750

    Purpose

    Expression of AURKA targeted to mitochondria

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Aurora A without the first 30AA + MTS of CoxVIII

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMOS001: CMV-Optopatch2_FCK

    Plasmid

    #62984

    Purpose

    Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology under CMV promoter

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    Optopatch2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

    Plasmid

    #78607

    Purpose

    A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2
    

    Use

    AAV and CRISPR

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short; U6

    Available since

    Sept. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pENTR-3xFLAG-YAP1-S127A

    Plasmid

    #42239

    Depositor

    William Pu

    Article

    von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Gateway

    Tags

    3xFLAG

    Expression

    Mutation

    Serine 127 changed to alanine

    Promoter

    none

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe-Puro-IKBalpha-wt

    Plasmid

    #15290

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    inhibitor of Kappa B alpha (NFKBIA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pICSL11060

    Plasmid

    #68264

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-XRCC1

    Plasmid

    #215142

    Purpose

    Expresses mCherry-XRCC1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    XRCC1 (XRCC1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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