175,351 results

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mito cpVenus control

Plasmid

#186792

Purpose

Identical to mito NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

Depositor

Michael Cohen

Article

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

mito cpVenus control

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_ACh3.0

Plasmid

#121923

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

Insert

GPCR activation based ACh sensor GRAB-ACh3.0

Use

AAV

Promoter

hSyn

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

Plasmid

#140002

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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DDR_MKOv4

Pooled Library

#140219

Purpose

Knockout library targeting human DNA damage response and DNA repair genes.

Depositor

Junjie Chen

Article

Su et al DNA Repair (Amst). 2020 Jan 16;87:102803

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only
miReporter-CAG

Plasmid

#82478

Purpose

microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

Insert

H2B-mCherry and H2B-Citrine. HygroR

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-AVP

Plasmid

#184600

Purpose

For expression of a fluorescent sensor for vasopressin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-AVP

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GRAB_g5-HT3.0

Plasmid

#208723

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0 in excitatory neurons

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0

Use

AAV

Promoter

CaMKII

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRetro-CAG-DsRedExpress-TVA

Plasmid

#235699

Purpose

Control plasmid expressing TVA and DsRed without G protein

Depositor

Guo-li Ming, Hongjun Song

Article

Sun et al Nature. 2025 Jan 16. doi: 10.1038/s41586

Insert

DsRedExpress, TVA800

Use

Retroviral

Promoter

CAG

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCI-Puro_hCOL4A4-Myc-His

Plasmid

#198093

Purpose

Express hCOL4A4 in mammalian cells

Depositor

Jeffrey Miner

Insert

COL4A4 (COL4A4 Human)

Tags

TEV-Myc-His

Expression

Mammalian

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag p38 gamma

Plasmid

#20353

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 gamma (Mapk12 Rat)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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miniSOG-H2B-C-10

Plasmid

#57769

Purpose

Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

H2B (H2BC11 Human)

Tags

miniSOG

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGWB602-RBCS-tagRFP

Plasmid

#213868

Purpose

Binary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

Insert

RIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)

Expression

Bacterial and Plant

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB508-cTP-mCherry

Plasmid

#213862

Purpose

Binary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

Insert

Plastid transit peptide fused mCherry-UnaG

Expression

Bacterial and Plant

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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delta-ZYA

Bacterial Strain

#52695

Purpose

MG1655 with Lac Operon removed from native locus

Depositor

Tanja Kortemme

Article

Eames et al Science. 2012 May 18;336(6083):911-5. do

Bacterial Resistance

None

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

Plasmid

#162335

Purpose

Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_ABEmax

Plasmid

#112095

Purpose

A:T-to-G:C base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

ABEmax

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pHG6

Plasmid

#188254

Purpose

Integrating plasmid contains the BT1 int/attP locus and the erythromycin-resistance gene for selection

Depositor

Hong Gao, Maggie Smith

Article

Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

Type

Empty backbone

Expression

Bacterial

Promoter

act1p/actII-orf4

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)

Plasmid

#162230

Purpose

Lentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaG17 GFP nanobody SynNotch tTA

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBT138b-proD

Plasmid

#138516

Purpose

P3e

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NOTUM-EF1a-TagRFP-2A-tet3G

Plasmid

#204188

Purpose

Doxycycline inducible lentiviral vector for human NOTUM overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NOTUM (NOTUM Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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