We narrowed to 32,648 results for: Eng

1. pAAV-Ef1a-FAS-GCaMP6f-WPRE

   Plasmid

   #141237

   Purpose

   AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)

   Depositor

   Guohong Cui

   Article

   Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

   Insert

   GCaMP6f
   

   Use

   AAV and Cre/Lox

   Tags

   6XHIS and Xpress

   Promoter

   Human elongation factor-1 alpha (EF-1 alpha)

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLINE1ΔORF2-scFvFc

   Plasmid

   #206021

   Purpose

   The ORF2 sequence was removed from pLINE1-scFvFc

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF2 sequence
   

   Expression

   Mammalian

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLINE1ΔORF1-2-scFvFc

   Plasmid

   #206022

   Purpose

   The ORF1 and ORF2 sequences were removed from pLINE1-scFvFc

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF1 and ORF2 sequences
   

   Expression

   Mammalian

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1-SHTN1S

   Plasmid

   #209561

   Purpose

   Mammalian expression of Shtn1 short isoform

   Depositor

   Sika Zheng

   Article

   Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

   Insert

   Shtn1 short isoform (4930506M07Rik Mouse)
   

   Expression

   Mammalian

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tol2-LyzC-PBD-GFP-U6ac-rac2 guides

   Plasmid

   #168251

   Purpose

   "label active Rac under Rac2 neutrophil-specific knockout background"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   PBD GFP
   

   Use

   Zebrafish expression

   Promoter

   LyzC

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides

   Plasmid

   #168254

   Purpose

   "label stable F-actin under Rac2 neutrophil-specific knockout background"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   Utrophin-GFP
   

   Use

   Zebrafish expression

   Promoter

   LyzC

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. puc19_N_tag

   Plasmid

   #186650

   Purpose

   Empty donor plasmid.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   N-terminal 3xFlag-3xHA Tag
   

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tol2-LyzC-PBD-GFP-U6ac-ctrl guides

   Plasmid

   #168252

   Purpose

   "label active Rac; control for neutrophil-specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   PBD GFP
   

   Use

   Zebrafish expression

   Promoter

   LyzC

   Available Since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides

   Plasmid

   #168253

   Purpose

   "label stable F-actin; control for neutrophil-specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   Utrophin-GFP
   

   Use

   Zebrafish expression

   Promoter

   LyzC

   Available Since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV V5-Tmem209

    Plasmid

    #175154

    Purpose

    Lentiviral expression of V5-tagged mouse Tmem209

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Tmem209 (Tmem209 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV Zdhhc6-V5

    Plasmid

    #175146

    Purpose

    Lentiviral expression of mouse Zdhhc6-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Zdhhc6 (Zdhhc6 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV Ptdss2-V5

    Plasmid

    #175143

    Purpose

    Lentiviral expression of mouse Ptdss2-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Ptdss2 (Ptdss2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV V5-mRnf185

    Plasmid

    #175126

    Purpose

    Lentiviral expression of V5-tagged mouse Rnf185

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Rnf185 (Rnf185 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV Tmem43-V5

    Plasmid

    #175111

    Purpose

    Lentiviral expression of mouse Tmem43-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Tmem43 (Tmem43 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-HA-DGCR8(+6)

    Plasmid

    #171541

    Purpose

    Encodes mutant human DGCR8 protein

    Depositor

    Yan Zeng

    Article

    Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    DGCR8 (DGCR8 Human)
    

    Expression

    Mammalian

    Mutation

    a six amino acid_TRLTEG_insertion in the L1 region

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. TMD18

    Plasmid

    #163673

    Purpose

    Expresses a ST6GAL1 mutant with 18 amino acids in its TMD in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    For this partial length ST chimera, two restricti…

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. TMD19

    Plasmid

    #163674

    Purpose

    Expresses a ST6GAL1 mutant with 19 amino acids in its TMD in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    For this partial length ST chimera, two restricti…

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PdnaK-IR2-IR2 GFP pZa

    Plasmid

    #170083

    Purpose

    GFP expression under the control of E. coli dnaK promoter engineered with double IR2 HAIR motif from M. tuberculosis

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PdnaK-IR2-IR2

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-uTEV1-SQSfl

    Plasmid

    #171010

    Purpose

    HiLITR protease with full-length SQS targeting information (ER)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-SQS(FL)
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    pTRE-tight (TetOn)

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pgRNA_CV2

    Plasmid

    #167165

    Purpose

    pgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.

    Depositor

    Alexander Loewer

    Article

    Sheng et al Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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