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pCMV-bpNLS-Bxb1 (EMK569)

Plasmid

#248208

Purpose

Bxb1 recombinase with N-terminal bpNLS, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-Bxb1

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

CMV and T7

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV- EF1α core -hKCNJ2-V77E-HA tag

Plasmid

#246996

Purpose

Can be used to generate AAV virus that will express human KCNJ2 V77E mutant with Ala115 HA tag from EF1α core promoter

Depositor

Ting Chen

Article

Chen et al Cell. 2025 Aug 14:S0092-8674(25)00857-8.

Insert

KCNJ2 (KCNJ2 Human)

Use

AAV

Tags

HA tag (Internal at Ala115)

Mutation

V77E

Promoter

EF1α

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-eGFP-HSV

Plasmid

#242769

Purpose

AAV transfer plasmid expressing eGFP-HSV under a CAG promoter.

Depositor

Andrew Payne

Article

Park et al bioRxiv 2025.09.26.678648

Insert

EGFP

Use

AAV

Tags

HSV

Promoter

CAG

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-eGFP-T7

Plasmid

#242778

Purpose

AAV transfer plasmid expressing eGFP-T7 under a CAG promoter.

Depositor

Andrew Payne

Article

Park et al bioRxiv 2025.09.26.678648

Insert

EGFP

Use

AAV

Tags

T7

Promoter

CAG

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pENTR223-FDX1

Plasmid

#251696

Purpose

Gateway-adapted FDX1 ORF

Depositor

Boyi Gan

Article

Lei et al Cancer Cell. 2025 Jun 9;43(6):1076-1092.

Insert

FDX1 ferredoxin 1 (FDX1 Human)

Use

Gateway Cloning

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pENTR221-SLC31A1

Plasmid

#251694

Purpose

Gateway-adapted SLC31A1 ORF

Depositor

Boyi Gan

Article

Lei et al Cancer Cell. 2025 Jun 9;43(6):1076-1092.

Insert

SLC31A1 solute carrier family 31 member 1 (SLC31A1 Human)

Use

Gateway Cloning

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pENTR221-BCL2

Plasmid

#251695

Purpose

Gateway-adapted BCL2 ORF

Depositor

Boyi Gan

Article

Lei et al Cancer Cell. 2025 Jun 9;43(6):1076-1092.

Insert

BCL2 BCL2 apoptosis regulator (BCL2 Human)

Use

Gateway Cloning

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GSDMD-Flag WT

Plasmid

#249726

Purpose

Express GSDMD WT in mammalian cells

Depositor

Hao Wu

Article

Du et al Nature. 2024 Jun;630(8016):437-446. doi:

Insert

gasdermin D (GSDMD Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pSIN4-DDX3X-IDR1+DDX6-ΔIDR

Plasmid

#240013

Purpose

Expresses human DDX6-DDX3X-IDR1+DDX6-ΔIDR fused to 3X Flag in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX3X-IDR1+DDX6-ΔIDR

Use

Lentiviral

Tags

3X Flag

Mutation

TTAAAAAAAGATAACATC

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti HBG site 2-ABE8e

Plasmid

#196099

Purpose

Expresses Lenti HBG site 2-ABE8e

Depositor

Xiahui Zhang

Article

Xue et al Nat Commun. 2023 Mar 3;14(1):1224. doi:

Insert

Lenti HBG site 2-ABE8e

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti HBG site 2-hyABE

Plasmid

#196100

Purpose

Expresses Lenti HBG site 2-hyABE

Depositor

Xiahui Zhang

Article

Xue et al Nat Commun. 2023 Mar 3;14(1):1224. doi:

Insert

Lenti HBG site 2-hyABE

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti HBG site 1-ABE8e

Plasmid

#196101

Purpose

Expresses Lenti HBG site 1-ABE8e

Depositor

Xiahui Zhang

Article

Xue et al Nat Commun. 2023 Mar 3;14(1):1224. doi:

Insert

Lenti HBG site 1-ABE8e

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti HBG site 1-hyABE

Plasmid

#196102

Purpose

Expresses Lenti HBG site 1-hyABE

Depositor

Xiahui Zhang

Article

Xue et al Nat Commun. 2023 Mar 3;14(1):1224. doi:

Insert

Lenti HBG site 1-hyABE

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pEvol-chKlacRS-IPYE

Plasmid

#232160

Purpose

A chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells.

Depositor

Nanxi Wang

Article

Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

Insert

chKlacRS-IPYE

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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PSIN4-3_Flag-ATP5MG

Plasmid

#232951

Purpose

Expresses ATP5MG

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

ATP5MG (ATP5MG Human)

Use

Lentiviral

Available Since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-ATP5MF

Plasmid

#232950

Purpose

Expresses ATP5MF

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

ATP5MF (ATP5MF Human)

Use

Lentiviral

Available Since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-ATP5PB

Plasmid

#232949

Purpose

Expresses ATP5PB

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

ATP5PB (ATP5F1 Human)

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaGIgG-4

Plasmid

#228998

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-4, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaGIgG-4

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaMIgG-9

Plasmid

#228993

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-9, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-9

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaMIgG-8

Plasmid

#228992

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-8, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-8

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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