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pGL4.23 D637G 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86024

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Lacks mucin-like domain: Changed aspartic acid 63…

Promoter

CMV IE

Available Since

April 14, 2018

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-mouse Gfi1-IRES-GFP

Plasmid

#91893

Purpose

Mammalian expression of mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Gfi1 (Gfi1 Mouse)

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
pCfB2795

Plasmid

#63650

Purpose

pCfB2795 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2802

Plasmid

#63655

Purpose

pCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2800

Plasmid

#63657

Purpose

pCfB2800 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 D500-630

Plasmid

#19841

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 D500-630 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 500-630

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 L272A

Plasmid

#19850

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 L272A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 272 to Alanine

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
p3xFLAG-MKL1 D381-506

Plasmid

#19853

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 381 to 506

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 S449A

Plasmid

#19842

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 S449A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Serine 449 to Alanine

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 T450A

Plasmid

#19843

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 T450A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Threonine 450 to Alanine

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pH6-TEV-spTorA-GFP(C)

Plasmid

#169041

Purpose

GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

H6-spTorA-GFP

Tags

6xHis, TEV cleavage site, and spTorA (signal pept…

Expression

Bacterial

Mutation

The mut3 GFP variant with 5 additional mutations …

Promoter

araBAD

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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YFP-tTA-ER-C3

Plasmid

#134568

Purpose

Expresses a YFP-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion protein

Depositor

Susan Janicki

Article

Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

Insert

tTA-ER

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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CFP-tTA-ER-C3

Plasmid

#134569

Purpose

Expresses a CFP-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion protein

Depositor

Susan Janicki

Article

Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

Insert

tTA-ER

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac-His6-Mps1-KD

Plasmid

#114064

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al Cell. 2008 Jan 25;132(2):233-46. doi: 10

Insert

Mps1-KD (TTK Human)

Tags

His6

Expression

Insect

Mutation

Mps1 KD (D664A)

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pScaf-8064.3

Plasmid

#111409

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 8064 bases in length (v3)

Depositor

Shawn Douglas

Article

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-GHI

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pScaf-8064.2

Plasmid

#111408

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 8064 bases in length (v2)

Depositor

Shawn Douglas

Article

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-BFJ

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCTD26-S>A10-17

Plasmid

#112826

Purpose

pSMF2 with all S to A mutations in only repeats 10-17.

Depositor

Stephen Fuchs

Article

Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

pSMF2 with all S to A mutations in only repeats 1…

Promoter

Native promoter

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCTD26-S>A2-9

Plasmid

#112825

Purpose

pSMF2 with all S to A mutations in only repeats 2-9.

Depositor

Stephen Fuchs

Article

Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

pSMF2 with all S to A mutations in only repeats 2…

Promoter

Native promoter

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCTD26-S>A18-25

Plasmid

#112827

Purpose

pSMF2 with all S to A mutations in only repeats 18-25.

Depositor

Stephen Fuchs

Article

Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

pSMF2 with all S to A mutations in only repeats 1…

Promoter

Native promoter

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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Ubiquitin (1-76; L71Y)

Plasmid

#110756

Purpose

Bacterial expression of human Ubiquitin (1-76; L71Y)

Depositor

David Komander

Article

Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

Insert

UBB (UBB Human)

Expression

Bacterial

Mutation

L71Y

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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