Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 322 results for: cas9 f cas9 r

Clear keywords
Showing: 281 - 290 of 322 results

  1. pHS1993

    Plasmid
    #239836
    Purpose
    OMEGAOff-KRK mammalian expression
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    DNMT3A-DNMT3L-OrufIscB-KRK-KRAB
    Tags
    HA and SV40 NLS
    Expression
    Mammalian
    Mutation
    Insertion of REC domain from NbaCas9-1 + E137K/E4…
    Promoter
    CMV
    Available Since
    July 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239836-plasmid-map-sequence-id-479108

    Enlarge

  2. pHS2096

    Plasmid
    #239835
    Purpose
    NovaIscB E. coli expression for recombinant purification
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    NovaIscB
    Tags
    14xHis-TwinStrep-bdSUMO
    Expression
    Bacterial
    Mutation
    Insertion of REC domain from NbaCas9-1 and swap o…
    Promoter
    T7
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239835-plasmid-map-sequence-id-479109

    Enlarge

  4. pCutamp

    Plasmid
    #140632
    Purpose
    Plasmid-curing in Escherichia coli by targeting the AmpR promoter
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140632-plasmid-map-sequence-id-306006

    Enlarge

  5. pHS1742

    Plasmid
    #239838
    Purpose
    OrufIscB-KRK mammalian expression
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    OrufIscB-KRK
    Tags
    Nucleoplasmin NLS-3xHA and SV40 NLS
    Expression
    Mammalian
    Mutation
    Insertion of REC domain from NbaCas9-1 + E137K/E4…
    Promoter
    CMV
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239838-plasmid-map-sequence-id-479115

    Enlarge

  6. pPPEDs

    Plasmid
    #162469
    Purpose
    protoplast test vector of pPPED
    Depositor
    Adam Bogdanove
    Article
    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do
    Inserts
    Cas9H840A
    attR gateway destination cassette
    Use
    Prime editing destination vector for transient ex…
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine
    Available Since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162469-plasmid-map-sequence-id-350377

    Enlarge

  10. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138147-plasmid-map-sequence-id-349848

    Enlarge

Showing: 281 - 290 of 322 results