-
Plasmid#173971PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertHuman minimal enhancer region 1 (min1) and coelacanth minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-EC1.45-human_min1-chicken_min2
Plasmid#173968PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertHuman minimal enhancer region 1 (min1) and chicken minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#7
Plasmid#173991PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #7 mutated
UseLuciferaseTagsExpressionMutationCoordinator motif mutation #7PromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-EC1.45-human_min1-platypus_min2
Plasmid#173967PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertHuman minimal enhancer region 1 (min1) and platypus minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2_3XCoordinatorMutant#5-6-7
Plasmid#173993PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motifs #5, #6 and #7 mutated
UseLuciferaseTagsExpressionMutation3X Coordinator motif mutation #5-6-7PromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_min1-2_3XCoordinatorMutant
Plasmid#173964PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertMinimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 3 mutated Coordinator motifs
UseLuciferaseTagsExpressionMutation3 mutated Coordinator motifsPromoterSV40 promoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-EC1.45-human_min1-mouse_min2
Plasmid#173965PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertHuman minimal enhancer region 1 (min1) and mouse minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-EC1.45-human_min1-lizard_min2
Plasmid#173969PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertHuman minimal enhancer region 1 (min1) and lizard minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMuSIC pool v1.0
Pooled Library#227193PurposeTwo-way barcodes from 18 fluorescent proteins for mammalian expression. Can be used for clone tracing, or for pairing with perturbations for single cell resolution in spectral detection measurements.DepositorExpressionMammalianAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-Archon1-KGC-GFP-ER2
Plasmid#115890PurposeAAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version). Using SV40 signal.DepositorInsertArchon1-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationPromoterEF1a(1.1kb short version)Available sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]
Plasmid#115893PurposeAAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorHas ServiceAAV8InsertArchon1-KGC-EGFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationPromoterSynAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-FLEX-rc [Archon1-KGC-GFP-ER2]
Plasmid#115891PurposeAAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorInsertArchon1-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationPromoterEF1α promoter (1.1kb short version)Available sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
NYESO beta/alpha into TRAC HDRT Source (pTR 169)
Plasmid#112021PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alphaDepositorInsertNYESO beta/alpha into TRAC HDRT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV[TetOn]-Puro-TRE3G>mAscl1
Plasmid#198755PurposeVector encodes the gene for murine Ascl1 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoterDepositorInsertAscl1 (Ascl1 Mouse)
UseLentiviralTagsExpressionMutationPromoterTRE3GAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
NYESO alpha/beta into TRBC1 HDRT Source (pTR 262)
Plasmid#112022PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-betaDepositorInsertNYESO alpha/beta into TRBC1 HDRDT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV[TetOn]-Puro-TRE3G>mNeurog2
Plasmid#198754PurposeVector encodes the gene for murine neurogenin-2 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoterDepositorInsertNeurogenin-2 (Neurog2 Mouse)
UseLentiviralTagsExpressionMutationPromoterTRE3GAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
NYESO alpha into TRAC HDRT Source (pTR 223)
Plasmid#112023PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alphaDepositorInsertNYESO alpha into TRAC HDRT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
NYESO beta into TRBC1 HDRT Source (pTR 277)
Plasmid#112024PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR-beta with 1G4 NYESO TCR-betaDepositorInsertNYESO beta into TRBC1 HDRT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sabatini/Lander Lab Ras synthetic lethal candidate focused human sgRNA library in lentiCRISPRv1
Pooled Library#92352Purposecontains 6,661 sgRNAs (comprising 499 intergenic control sgRNAs and 6,162 sgRNAs targeting 132 control and candidate Ras synthetic lethal genes at up to 50 sgRNAs per gene)DepositorSpeciesHomo sapiensAvailable sinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
RAB11A-GFP HDRT Source (pTR 143)
Plasmid#112012PurposeDNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFPDepositorInsertRAB11A-GFP HDRT (RAB11A Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
CD4-C-GFP HDRT Source (pTR 152)
Plasmid#112018PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFPDepositorInsertCD4-GFP HDRT (CD4 Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
CLTA-GFP HDRT Source (pTR 153)
Plasmid#112016PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFPDepositorInsertCLTA-GFP HDRT (CLTA Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
RAB11A-mCherry HDRT Source (pTR 144)
Plasmid#112013PurposeDNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherryDepositorInsertRAB11A-mCherry HDRT (RAB11A Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
CLTA-mCherry HDRT Source (pTR 177)
Plasmid#112017PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherryDepositorInsertCLTA-mCherry HDRT (CLTA Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
BATF-GFP HDRT Source (pTR 146)
Plasmid#112015PurposeDNA sequence source for amplifying an HDR template to tag endogenous human BATF gene with GFPDepositorInsertBATF-GFP HDRT (BATF Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
RAB11A-BFP HDRT Source (pTR 145)
Plasmid#112014PurposeDNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFPDepositorInsertRAB11A-BFP HDRT (RAB11A Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
CD4-C-BFP HDRT Source (pTR 176)
Plasmid#112020PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFPDepositorInsertCD4-BFP HDRT (CD4 Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
CD4-C-mCherry HDRT Source (pTR 175)
Plasmid#112019PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherryDepositorInsertCD4-mCherry HDRT (CD4 Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
PG13-mCherry
Plasmid#195291PurposemCherry reporter containing 13 copies of the p53-binding consensus sequenceDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-hNGN2
Plasmid#172115PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expressionInsertsUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only