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Plasmid#169395PurposeCell-free/mammalian expression vector for SARS-COV-2 virus ORF8 with N-term eGFPDepositorInsertSARS-COV-2 _ORF8 (ORF8 SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2 _NSP1
Plasmid#169376PurposeCell-free/mammalian expression vector for SARS-COV-2 virus NSP1 with N-term eGFPDepositorInsertSARS-COV-2 _NSP1
UseCell-free expression vectorTagsHis and eGFPExpressionMutationCodon optimized for Saccharomyces cerevisiaePromoterT7- CMV/CAGAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_ORF6
Plasmid#169392PurposeCell-free/mammalian expression vector for SARS-COV-2 virus ORF6 with N-term eGFPDepositorInsertSARS-COV-2 _ORF6 (ORF6 SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_ORF3a
Plasmid#169390PurposeCell-free/mammalian expression vector for SARS-COV-2 virus ORF3a with N-term eGFPDepositorInsertSARS-COV-2 _ORF3a (ORF3a SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationCodon optimized for Saccharomyces cerevisiaePromoterT7- CMV/CAGAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_ORF7b
Plasmid#169394PurposeCell-free/mammalian expression vector for SARS-COV-2 virus ORF7b with N-term eGFPDepositorInsertSARS-COV-2 _ORF7b (ORF7b SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationCodon optimized for Saccharomyces cerevisiaePromoterT7- CMV/CAGAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_ORF7a
Plasmid#169393PurposeCell-free/mammalian expression vector for SARS-COV-2 virus ORF7a with N-term eGFPDepositorInsertSARS-COV-2 _ORF7a (ORF7a SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationCodon optimized for Saccharomyces cerevisiaePromoterT7- CMV/CAGAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 H2B-mIFP T2A Mpro (wt)
Plasmid#163079PurposeExpresses wild type Mpro (active) and a near-infrared fluorescent protein mIFP fused to H2BDepositorInsertWild type Mpro of SARS-CoV-2 and H2B-mIFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 H2B-mIFP T2A Mpro (C145A)
Plasmid#163080PurposeExpresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2BDepositorInsertInactive Mpro mutant C145A and H2B-mIFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_RBD -N501Y
Plasmid#169407PurposeCell-free/mammalian expression vector for SARS-COV-2 mutated RBD domain (N510Y, UK strain) of S protein with N-term eGFPDepositorInsertSARS-COV-2 _RBD (N501Y) (S SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationN501YPromoterT7- CMV/CAGAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_Human-ACE2
Plasmid#169406PurposeCell-free/mammalian expression vector for Human ACE2 receptor with N-term eGFPDepositorInsertHuman ACE2 receptor (ACE2 Human)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCell-Free_KA1_SARS-COV-2_S-frag3
Plasmid#169403PurposeCell-free/mammalian expression vector for SARS-COV-2 virus N-term fragment of S protein after the furin cleavag with N-term eGFPDepositorInsertN-term frag. S protein after the furin cleavag (N SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_S-frag5
Plasmid#169404PurposeCell-free/mammalian expression vector for SARS-COV-2 virus C-terminal ectodomain of S protein from the furin cleavag with N-term eGFPDepositorInsertC-term frag. S protein after the furin cleavag (S SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQC
Plasmid#221134PurposeContains [PibpA-gfpASV] and [PcpxPmcherryASV]OPT genetic modules in the pSEVA631(Spec) backbone, separated by the secG leuU terminatorDepositorInserts[PibpA-gfpASV]
[PcpxPmcherryASV] OPT
UseTagsExpressionBacterialMutationPromotercpxP and ibpAAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK187
Plasmid#156423PurposeInsect cell expression of 3a-sfGFP, needs to be introduced into baculoviral bacmidDepositorInsertSARS-CoV-2 3a protein
UseTagssfGFP-HisExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK186
Plasmid#156422PurposeMammalian expression of 3a-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cellsDepositorInsertSARS-CoV-2 3a protein
UseTagsEGFP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK193
Plasmid#156424PurposeInsect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmidDepositorInsertSARS-CoV-2 3a protein (Q57H)
UseTagssfGFP-HisExpressionInsectMutationQ57HPromoterPolyhedrinAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK198
Plasmid#156425PurposeInsect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmidDepositorInsertSARS-CoV-2 3a protein (aa 42-275)
UseTagssfGFP-HisExpressionInsectMutationaa 42-275PromoterPolyhedrinAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSKA413
Plasmid#80381PurposeConstitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.DepositorInsertsccaS (complement)
ccaR (complement)
gfpmut3
UseSynthetic Biology; OptogeneticsTagsFLAG tagExpressionBacterialMutationPromoterccaR and cpcG2Δ59Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK202
Plasmid#156426PurposeMammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cellsDepositorInsertSARS-CoV-2 3a protein (aa 42-275)
UseTagsEGFP-HisExpressionMammalianMutationaa 42-275PromoterCMVAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTG8-Amp-FRT
Plasmid#128277PurposeTIP-LITer2.0-KRAS(G12V) gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP-P2A-KRAS(G12V)-P2AGFPDepositorInsertsTetR-LOV-TIP
EGFP
KRAS(G12V)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only