Addgene

We narrowed to 11,227 results for: HAL;

Showing: 561 - 580 of 11227 results

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pHTC-HaloTag_ACTB:1-375

Plasmid

#210971

Purpose

Mammalian expression of full-length human ACTB. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

ACTB:M1-F375 (ACTB Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_DCAF12:1-453

Plasmid

#210967

Purpose

Mammalian expression of full-length human DCAF12. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF12:M1-S453 (DCAF12 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

R131Q natural variant (VAR_035322)

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_ACTB:1-375

Plasmid

#210970

Purpose

Mammalian expression of full-length human ACTB. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

ACTB:M1-F375 (ACTB Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAdEasy-hALDOA D34S

Plasmid

#210813

Purpose

To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Adenoviral

Tags

HA

Expression

Mutation

residues 34 of ALDOA mutated to serine

Promoter

CMV

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hALDOA K230A

Plasmid

#210811

Purpose

To block the formation of the initial Schiff base with FBP

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Tags

HA

Expression

Mammalian

Mutation

residues 230 of ALDOA mutated to alanine

Promoter

CMV

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBoBi-hALDOA K230A

Plasmid

#210808

Purpose

To block the formation of the initial Schiff base with FBP

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

residues 230 of ALDOA mutated to alanine

Promoter

CMV

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBoBi-hALDOA D34S

Plasmid

#210807

Purpose

To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

residues 34 of ALDOA mutated to serine

Promoter

CMV

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-tagged Sox17FNV

Plasmid

#206391

Purpose

Expresses Halo-tagged mouse SOX17FNV in mammalian cells, for single-molecule tracking.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

Sox17FNV (Sox17 Mouse)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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p10xUAS-WHaloCaMP1a-EGFP

Plasmid

#205314

Purpose

Expression of WHaloCaMP1a-EGFP in Drosophila using GAL4 driver

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT001-HaloTag-1x FLAG

Plasmid

#202492

Purpose

Express "soluble" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Use

Tags

1x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT004-1x FLAG-HaloTag-G

Plasmid

#202495

Purpose

Express "G" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Use

Tags

1x FLAG and Geranylgeranyl site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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NLS-Halo-HSF2

Plasmid

#200198

Purpose

Expression of full length HSF2 with SV40 NLS

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

3X SV40 NLS-Halo-HSF2 (Hsf2 Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-HaloTag

Plasmid

#181953

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

HaloTag

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-blast-3HaloTag

Plasmid

#179827

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Keith Gull, Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3x HaloTag

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-g418-3HaloTag

Plasmid

#179829

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Keith Gull, Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3x HaloTag

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad-HaloGFP-Ca0.5

Plasmid

#198632

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Ca0.5

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad-HaloGFP-Ca0.2

Plasmid

#198631

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Ca0.2

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad-HaloGFP-Ca1

Plasmid

#198633

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Ca1

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
hp-50%GC-Left-half-120bp

Plasmid

#169204

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50%GC-Left-half-120bp-hairpin

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
hp-50%GC-Right-half-120bp

Plasmid

#169205

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50%GC-Right-half-120bp-hairpin

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge