We narrowed to 9,267 results for: Pol;

1. pAAV-CAG-EGFP barcode-10-SV40 polyA

   Plasmid

   #190874

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode10
   

   Use

   AAV

   Mutation

   WT

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. RNA polymerase beta prime subunit FLAG

   Plasmid

   #102337

   Purpose

   Targeting plasmids for replacing the S. elongatus PCC 7942 genomic copy of Synpcc7942_1524 (Beta prime subunit of RNA polymerase) with a C-terminal FLAG tagged variant.

   Depositor

   Erin O'Shea

   Article

   Piechura et al Elife. 2017 Dec 14;6. pii: 32032. doi: 1

   Insert

   RNAP Beta Prime Subunit with C-terminal FLAG TAG
   

   Tags

   3x FLAG with 3X GSGS linker

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.CMV.SV40.THBS1-deltaTSR3-CTD-HA.SV40(polyA)

   Plasmid

   #155194

   Purpose

   Expresses truncated (AA 1-690) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Thrombospondin-1 (Thbs1 Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Truncated sequence of THBS1 (expresses Amino Acid…

   Promoter

   CMV

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. IX301: pMVP (L3-L2) myc epitope tag + polyA

   Plasmid

   #121753

   Purpose

   pMVP L3-L2 entry plasmid, contains myc epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   myc epitope tag + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. IX601: pMVP (L3-L2) FLAG epitope tag + polyA

   Plasmid

   #121751

   Purpose

   pMVP L3-L2 entry plasmid, contains FLAG epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   FLAG epitope tag + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS NL4-3 envFS eGFP Δgag/pol

   Plasmid

   #101343

   Purpose

   Deletion from the start of gag until 229 bases before the cPPT

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Mutation

   delta GagPol

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1-fDIO-mNeon-WPRE-PolyA

   Plasmid

   #121546

   Purpose

   Super-bright fluorescence protein for tracking and visualization

   Depositor

   Matthias Prigge

   Insert

   cre-dependent mNeon
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. C513-E07: attB5r-<p10p-polyhedrinp>-attB1r

   Plasmid

   #162949

   Purpose

   Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)
   

   Use

   Synthetic Biology

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. YIp211-P30-His-pol30(K127/164R)-3'

   Plasmid

   #99549

   Purpose

   Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; URA3 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   POL30 (POL30 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   K127R, K164R

   Promoter

   POL30

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMJO-6 (minus T7 RNA polymerase binding site)

    Plasmid

    #31991

    Depositor

    Mark Osborn

    Article

    Osborn et al Hum Gene Ther. 2011 Jun 13.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3E-hGPR56 e1m promoter perisylvian polymicrogyria

    Plasmid

    #52299

    Purpose

    Reporter for mutated human GPR56 exon 1m promoter activity. It contains a 15-bp deletion in a conserved noncoding element. The mutated human GPR56 e1m promoter was inserted into pGL3E.

    Depositor

    Christopher A Walsh

    Article

    Bae et al Science. 2014 Feb 14;343(6172):764-8. do

    Insert

    ADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
    

    Use

    Luciferase

    Mutation

    15-bp deletion in the promoter

    Promoter

    Human GPR56 e1m promoter

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-G1B3-GFP-WPRE-miR124T-SYNpolyA

    Plasmid

    #245081

    Purpose

    Fluorescent reporter gene, with miRNA-124 target sequence to repress transgene expression in neurons

    Depositor

    Nicole Déglon

    Insert

    EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human G1B3 promoter

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVss-G1B3-nlsCre-WPRE-miR124T-SYNpolyA

    Plasmid

    #245080

    Purpose

    CRE recombinase with miRNA-124 target sequence to repress transgene expression in neurons

    Depositor

    Nicole Déglon

    Insert

    nls-Cre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human G1B3 promoter

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. L4315 IL-10Ra CTEVp chain in PolyTX-mTagBFP2

    Plasmid

    #244171

    Purpose

    Constitutive expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-VP64-ZF6a

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    MESA CTEVp chain with human IL-10Ra SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (IL10RA Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    IL10Ra signal peptide - 3xFLAG

    Expression

    Mammalian

    Mutation

    CTEVp_190K

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. L4804 VEGFR1 CTEVp chain in PolyTX-mTagBFP2

    Plasmid

    #244174

    Purpose

    Constitutive expression of a MESA CTEVp chain encoding (N to C): VEGFR1SS-3xFLAG-VEGFR1ECD-VEGFR1TMD-CTEVp(190K)-PRS(M)-VP64-ZF6

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    MESA CTEVp chain with human VEGFR1 SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (FLT1 Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    VEGFR1 signal peptide - 3xFLAG

    Expression

    Mammalian

    Mutation

    CTEVp_190K

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. L4207 TNFR2 NTEVp chain in PolyTX-mNeonGreen

    Plasmid

    #244175

    Purpose

    Constitutive expression of a MESA NTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-NTEVp(75S)

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    MESA NTEVp chain with human TNFR2 SS and ECD, murine CD28 TMD, and NTEVp (75S) (TNFRSF1B Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    TNFR2 signal peptide - 3xFLAG

    Expression

    Mammalian

    Mutation

    NTEVp_75S

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. L4342 TNFR1 CTEVp chain in PolyTX-mTagBFP2

    Plasmid

    #244178

    Purpose

    Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-CTEVp(75S)-PRS(M)-VP64-ZF6

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    MESA CTEVp chain with human TNFR1 SS, ECD (no MMP site) and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (TNFRSF1A Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    TNFR1 signal peptide - 3xFLAG

    Expression

    Mammalian

    Mutation

    CTEVp_190K

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. L4219 TNFR2 CTEVp chain in PolyTX-mTagBFP2

    Plasmid

    #244177

    Purpose

    Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-CTEVp(190K)-PRS(M)-VP64-ZF6

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    MESA CTEVp chain with human TNFR2 SS and ECD, murine CD28 TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (TNFRSF1B Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    TNFR2 signal peptide - 3xFLAG

    Expression

    Mammalian

    Mutation

    CTEVp_190K

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNAintron-CHIKV-Structural polyprotein with Nluc tagged E2

    Plasmid

    #215699

    Purpose

    Produces the Chikungunya virus structural polyprotein Capsid-E3-NLucE2-6K-E1

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV structural polyprotein Capsid-E3-NLucE2-6K-E1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. Polymerase expression - pCMV-ePhi29(+exo) (LM2995)

    Plasmid

    #208966

    Purpose

    Unfused ePhi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    ePhi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…

    Promoter

    CMV and T7

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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