Addgene

We narrowed to 6,531 results for: human c myc

Showing: 561 - 580 of 6531 results

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AAVS1_Puro_hPGK1_EBFP_Donor

Plasmid

#178089

Purpose

HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EBFP (T65S/F145Y)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human PGK1

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPU-Empty-miR30-L1221

Plasmid

#132940

Purpose

All-in-one control rescue-control shRNA knockdown vector

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

GFP

Use

Lentiviral and RNAi

Tags

HA

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pFUPI

Plasmid

#80279

Purpose

A bicistronic lentiviral vector used to stably express cDNAs.

Depositor

Jeremy Luban

Article

Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

Insert

Puro resistance cassette, IRES, cloning site

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pFUPI AoT5Cyp

Plasmid

#80280

Purpose

Expresses Aotus TRIM5 CypA fusion

Depositor

Jeremy Luban

Article

Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

Insert

TRIM5 CypA fusion

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Hyg-FHIT

Plasmid

#16410

Depositor

Bert Vogelstein

Article

Kohli et al Nucleic Acids Res. 2004 . 32(1):e3.

Insert

FHIT Hyg targeting (FHIT Human)

Use

AAV; Adeno-associated viral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pT7-EGFP-C1-HsTNRC6A

Plasmid

#25035

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-STRA8

Plasmid

#222585

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of STRA8, with hygromycin resistance

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

STRA8 (STRA8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDOCKING-Neo

Plasmid

#31441

Depositor

Gerhart Ryffel

Article

Waldner et al BMC Res Notes. 2011 Oct 18;4:420.

Insert

ECFP-neomycin

Use

Cre/Lox; Phic31/attp

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pTRIPZ

Plasmid

#206981

Purpose

empty pTRIPZ vector for cloning of doxycycline-inducible gene expression

Depositor

Ming Chen

Article

Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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PB-PEmax

Plasmid

#187893

Purpose

All-in-one prime editor piggyBac transposon with PEmax

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

BPSV40 NLS and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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PB-PEmax NG

Plasmid

#187894

Purpose

All-in-one prime editor piggyBac transposon with PEmax, NG variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_KK_NG-MMLV-RT_co-P2A-PAC_dTK

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

BPSV40 NLS and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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PB-PEmax SpG

Plasmid

#187895

Purpose

All-in-one prime editor piggyBac transposon with PEmax, SpG variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

BPSV40 NLS and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pIRES2-hPAC-FLAG

Plasmid

#129494

Purpose

Expresses human TMEM206 tagged at the C-terminus with FLAG

Depositor

Zhaozhu Qiu

Article

Yang et al Science. 2019 Apr 26;364(6438):395-399.

Insert

human PAC tagged with FLAG tag at the C-terminal end (PACC1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pHDR_DDX4-T2A-tdTomato-puroTK

Plasmid

#222519

Purpose

Homology directed repair template for knocking in tdTomato reporter to DDX4.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

tdTomato

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHDR_REC8-T2A-mGreenLantern-puroTK

Plasmid

#222521

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to REC8.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

mGreenLantern

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pRECLV103_PGBD5_B

Plasmid

#168053

Purpose

Lentiviral expression vector for fusion of GFP with human PGBD5, NCBI: NM_001258311.1

Depositor

Alex Kentsis

Article

Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

Insert

PGBD5 (PGBD5 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Plk1(829)

Plasmid

#39845

Depositor

Jonathon Pines

Article

Lindon et al J Cell Biol. 2004 Jan 19;164(2):233-41.

Insert

Plk1 (PLK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
FR_DsRed2

Plasmid

#31444

Depositor

Gerhart Ryffel

Article

Waldner et al BMC Res Notes. 2011 Oct 18;4:420.

Insert

DsRed2

Use

Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCR2.1-KRAS-G12V

Plasmid

#129091

Purpose

KI KRAS G12V mutation into human KRAS locus

Depositor

Toshiro Sato

Article

Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

Insert

KRAS(G12V) (KRAS Human)

Use

Homologous recombination donor plasmid

Tags

Expression

Mutation

G12V

Promoter

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-F-Med26-NTD R61A K62A

Plasmid

#49256

Purpose

Mammalian expression of flag-tagged human Med26-NTD R61A K62A

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med 26 (MED26 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

NTD R61A K62A

Promoter

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only