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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,499 results for: multiplex

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Showing: 561 - 580 of 3499 results

  3. pX330A_D10A-1x7

    Plasmid
    #58777
    Purpose
    Expresses Cas9 nickase and gRNA
    Depositor
    Takashi Yamamoto
    Publication
    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Promoter
    CBh
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58777-plasmid-map-sequence-id-192734

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  4. pAAV_hSyn-PdCO-mScarlet-WPRE (AAV1)

    Viral prep
    #198510-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV_hSyn-PdCO-mScarlet-WPRE (#198510). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Publication
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    Syn
    Tags
    mScarlet
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only

  7. pcDNA3-RAB-EKARev(T/A)

    Plasmid
    #118475
    Purpose
    Negative-control mutant for RAB-EKARev biosensor.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB-EKARev(T/A)
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    May 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    118475-plasmid-map-sequence-id-243129

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  8. pCMV/MASC_(GLuc)_TOP1

    Plasmid
    #68439
    Purpose
    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOP1 construct
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68439-plasmid-map-sequence-id-120217

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  12. pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP

    Plasmid
    #169333
    Purpose
    Expression of HaloTag9 at the mitochondrial surface of mammalian cells
    Depositor
    Kai Johnsson
    Publication
    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOMM20-HaloTag9 (TOMM20 Human)
    Tags
    HaloTag9
    Expression
    Mammalian
    Mutation
    HaloTag9 = HaloTag7-Q165H-P174R
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    169333-plasmid-map-sequence-id-333241

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  15. pAAV-UBC-S3-ExRaiAKAR

    Plasmid
    #160724
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.
    Depositor
    Edward Boyden
    Publication
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S3-ExRaiAKAR
    Use
    AAV
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160724-plasmid-map-sequence-id-315754

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  16. pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE (AAV1)

    Viral prep
    #202199-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE (#202199). In addition to the viral particles, you will also receive purified pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE plasmid DNA. Synapsin-driven, Cre-dependent expression of bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized opsin PdCO. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Publication
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    Syn
    Tags
    EGFP
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  19. hCMV-IE1:ELuc

    Plasmid
    #118062
    Purpose
    Transcriptional unit for constitutive expression of ELuc in mammalian cells
    Depositor
    Koen Venken
    Publication
    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CMV:ELuc:bGH
    Use
    Luciferase and Synthetic Biology; Assembly of gb2…
    Expression
    Mammalian
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118062-plasmid-map-sequence-id-240491

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  20. hCMV-IE1:FLuc

    Plasmid
    #118063
    Purpose
    Transcriptional unit for constitutive expression of FLuc in mammalian cells
    Depositor
    Koen Venken
    Publication
    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CMV:FLuc:bGHF
    Use
    Luciferase and Synthetic Biology; Assembly of gb2…
    Expression
    Mammalian
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118063-plasmid-map-sequence-id-240492

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Showing: 561 - 580 of 3499 results