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pAAV-CaMKII-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Plasmid

#165493

Purpose

Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Use

AAV

Tags

darkVenus

Expression

Mammalian

Promoter

CaMKII

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-30uDelta13V Venus-Y67G

Plasmid

#18016

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu D154A Delta13V Venus-Y67G

Tags

ECFP and Venus-Y67G

Expression

Mammalian

Mutation

mglB-D154A; non-functional Venus

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-30uDelta13V Venus-Y67G

Plasmid

#18016

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu D154A Delta13V Venus-Y67G

Tags

ECFP and Venus-Y67G

Expression

Mammalian

Mutation

mglB-D154A; non-functional Venus

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE-600n

Plasmid

#13537

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Tags

ECFP and Venus

Expression

Bacterial

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPE-1m

Plasmid

#13544

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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Venus-30aa-Noxa-pEGFP-C1

Plasmid

#166762

Purpose

To increase the length of the linker between Venus and Noxa proteins

Depositor

David Andrews

Insert

Noxa (PMAIP1 Human)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE-600n

Plasmid

#13537

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Tags

ECFP and Venus

Expression

Bacterial

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPE-1m

Plasmid

#13544

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Plasmid

#165492

Purpose

Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Use

AAV

Tags

darkVenus

Expression

Mammalian

Promoter

hSyn1

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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Scarlet I-FRET 10-Venus

Plasmid

#139366

Purpose

FRET Standard

Depositor

Richard Day

Article

Day et al PLoS One. 2021 Dec 23;16(12):e0261660. d

Insert

Scarlet 10AA Venus

Expression

Mammalian

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-Venus-iGluSnFR.A184S

Plasmid

#106201

Purpose

Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184S

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Plasmid

#165492

Purpose

Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Use

AAV

Tags

darkVenus

Expression

Mammalian

Promoter

hSyn1

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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C5A

Plasmid

#27799

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

Cerulean-5-Amber

Use

Fret donor control

Tags

Amber and Cerulean

Expression

Mammalian

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pAAV.hSynapsin.SF-Venus-iGluSnFR.A184S

Plasmid

#106177

Purpose

Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184S

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

Promoter

hSynapsin

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-Venus-iGluSnFR.A184S

Plasmid

#106201

Purpose

Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184S

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
C5A

Plasmid

#27799

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

Cerulean-5-Amber

Use

Fret donor control

Tags

Amber and Cerulean

Expression

Mammalian

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pZH051

Plasmid

#47311

Purpose

Expresses Tsr-Venus-Ubiquitin-CI

Depositor

Jie Xiao

Article

Hensel et al Nat Struct Mol Biol. 2012 Aug;19(8):797-

Insert

wild type immunity region of lambda phage with tsr-venus-ub-cI

Tags

CI

Expression

Bacterial

Promoter

PR; PRM

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pZH051

Plasmid

#47311

Purpose

Expresses Tsr-Venus-Ubiquitin-CI

Depositor

Jie Xiao

Article

Hensel et al Nat Struct Mol Biol. 2012 Aug;19(8):797-

Insert

wild type immunity region of lambda phage with tsr-venus-ub-cI

Tags

CI

Expression

Bacterial

Promoter

PR; PRM

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
Scarlet I-FRET 10-Venus

Plasmid

#139366

Purpose

FRET Standard

Depositor

Richard Day

Article

Day et al PLoS One. 2021 Dec 23;16(12):e0261660. d

Insert

Scarlet 10AA Venus

Expression

Mammalian

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-Venus-iGluSnFR.A184V

Plasmid

#106202

Purpose

Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184V

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge