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TFORF2818

Plasmid

#142999

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GON4L (GON4L Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC1664 - pAAV SYN1 mGas6(delta)-Myc-DDK

Plasmid

#202541

Purpose

An adeno-associated viral vector expressing murine Gas6 with deletion of (F50-E275) fused Myc and DDK epitopes from a synapsin promoter

Depositor

Christopher Richie

Article

Owlett et al J Neuroinflammation. 2022 Feb 7;19(1):38

Insert

Gas6 (Gas6 Mouse)

Use

AAV

Tags

Myc-DDK

Mutation

Deletion of F50-E275

Promoter

SYN1

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-VTCN1-COMP5AP-AviTag-9xHis

Plasmid

#157251

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

VTCN1 (VTCN1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS_L4139P-eGFP-3HA

Plasmid

#108404

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Human)

Tags

3xHA and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS_V4146I-eGFP

Plasmid

#108405

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely non-pathogenic variant.

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Tags

3xHA and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS_L4137P-eGFP

Plasmid

#108403

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Tags

3xHA and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS-eGFP

Plasmid

#108402

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Tags

3xHA and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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CDK11B gRNA (BRDN0001146432)

Plasmid

#77063

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK11B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK11B (CDK11B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK11B gRNA (BRDN0001146757)

Plasmid

#77064

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK11B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK11B (CDK11B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K4 gRNA (BRDN0001148639)

Plasmid

#76510

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K4 (MAP3K4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K4 gRNA (BRDN0001148603)

Plasmid

#76511

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K4 (MAP3K4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ETNK2 gRNA (BRDN0001147513)

Plasmid

#76168

Purpose

3rd generation lentiviral gRNA plasmid targeting human ETNK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ETNK2 (ETNK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ETNK2 gRNA (BRDN0001149306)

Plasmid

#76169

Purpose

3rd generation lentiviral gRNA plasmid targeting human ETNK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ETNK2 (ETNK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ETNK2 gRNA (BRDN0001149182)

Plasmid

#76166

Purpose

3rd generation lentiviral gRNA plasmid targeting human ETNK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ETNK2 (ETNK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ETNK2 gRNA (BRDN0001149328)

Plasmid

#76167

Purpose

3rd generation lentiviral gRNA plasmid targeting human ETNK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ETNK2 (ETNK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCE-hSK

Plasmid

#41814

Purpose

Non-integrating (episomal) expression of human SOX2 and KLF4

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

SOX2, KLF4 (SOX2, KLF4 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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mCherry-PCNA

Plasmid

#215117

Purpose

Expresses mCherry-PCNA in mammalian cells from a lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PCNA (PCNA Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-LIG1

Plasmid

#215131

Purpose

Expresses mCherry-LIG1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

LIG1 (LIG1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-LIG3

Plasmid

#215143

Purpose

Expresses mNeonGreen-LIG3 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

LIG3 (LIG3 Human)

Use

Retroviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-APEX1

Plasmid

#215126

Purpose

Expresses mCherry-APEX1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

APEX1 (APEX1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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