We narrowed to 7,293 results for: Ank

1. pES134

   Plasmid

   #127551

   Purpose

   Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

   Depositor

   Emmanuele Severi, Gavin Thomas

   Article

   Wu et al Microbiology (Reading). 2024 Mar;170(3).

   Insert

   F3-flanked spectinomycin resistance cassette
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pgRNA-backbone

   Plasmid

   #172517

   Purpose

   Yeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertion

   Depositor

   Leonid Kruglyak

   Article

   Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

   Insert

   gRNA cassette
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-attBr-ZsGreen-attP

   Plasmid

   #51265

   Purpose

   Bxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   attBr-ZsGreen-attP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. WNV NS2B-NS3 protease (based on PDB 2YOL, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion protein

   Plasmid

   #204798

   Purpose

   Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   West Nile NS2B-NS3 fusion
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pTV-Nanog Bgeo

   Plasmid

   #13837

   Depositor

   Shinya Yamanaka

   Article

   Mitsui et al Cell. 2003 May 30. 113(5):631-42.

   Insert

   IRES-Bgeo-pA cassette flanked by Nanog homologous regions (Nanog Mouse)
   

   Use

   Beta-gal

   Expression

   Mammalian

   Mutation

   None

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

6. YPRCΔ15α1

   Plasmid

   #101712

   Purpose

   GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Type

   Empty backbone

   Use

   Yeast expression

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-hDAGLb-V5

   Plasmid

   #87675

   Purpose

   Expresses human DAGLb protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal

   Depositor

   Pat Doherty

   Insert

   Human Diacylglycerol Lipase Beta (DAGLB Human)
   

   Tags

   HA and V5 tag

   Expression

   Mammalian

   Mutation

   Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…

   Promoter

   T7

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. YPRCΔ15Ω1

   Plasmid

   #101710

   Purpose

   GoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Type

   Empty backbone

   Use

   Yeast expression

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-attBr-Cre-rc-attP

   Plasmid

   #51264

   Purpose

   Component of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientation

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   attBr-Cre-rc-attP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. Plas-gRNA-LEU2

    Plasmid

    #157656

    Purpose

    GFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequence

    Depositor

    Frank Albert

    Article

    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

    Insert

    GFP-gRNA
    

    Use

    Insert storage (replicative in e. coli)

    Tags

    GFP-gRNA

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-roxSTOProx-LynEGFP

    Plasmid

    #133923

    Purpose

    Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Dre-based recombination

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    LynEGFP
    

    Expression

    Mammalian

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLP-hSox2-ires-gfp

    Plasmid

    #49391

    Purpose

    mammalian bicistronic expression of human Sox2 and EGFP

    Depositor

    Randall McKinnon

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Cre/Lox; Acceptor vector

    Tags

    6xHN, EGFP, and IRES

    Expression

    Mammalian

    Mutation

    TGA (stop) to CCA (Pro) at nt 1027 with respect t…

    Promoter

    CMV

    Available Since

    Dec. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pHD-mW-attPCC-FRT (pTL717)

    Plasmid

    #115158

    Purpose

    Vector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.

    Depositor

    Tobias Langenhan

    Article

    Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

    Insert

    mW attP-CC FRT
    

    Expression

    Insect

    Available Since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTV-Nanog Hyg

    Plasmid

    #13836

    Depositor

    Shinya Yamanaka

    Article

    Mitsui et al Cell. 2003 May 30. 113(5):631-42.

    Insert

    IRES-Hyg-pA cassette flanked by Nanog homologous regions (Nanog Mouse)
    

    Use

    Beta-gal

    Expression

    Mammalian

    Mutation

    None

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pENTR-THT III

    Plasmid

    #55791

    Purpose

    GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Gentamycin resistance

    Depositor

    Stephan Geley

    Article

    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

    Type

    Empty backbone

    Use

    RNAi; Gateway

    Expression

    Mammalian

    Promoter

    modified H1 RNA gene

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBH31(gfcD)

    Plasmid

    #176199

    Purpose

    Encodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasm

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcD
    

    Tags

    pelC leader-6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBH31(gfcD-10xHis)

    Plasmid

    #176200

    Purpose

    Encodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasm

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcD
    

    Tags

    pelC leader-10xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC0.167

    Plasmid

    #119636

    Purpose

    Level 0 Part. Insertion site

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    6803 NS2 Up Flank
    

    Use

    Synthetic Biology

    Mutation

    non functional/large non-specific insertion

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC0.168

    Plasmid

    #119635

    Purpose

    Level 0 Part. Insertion site

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    6803 NS2 Down Flank
    

    Use

    Synthetic Biology

    Mutation

    bp 630 A to T

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pReporter_13_switchBP

    Plasmid

    #62848

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). attP upstream of attB in this plasmid.

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_13_switchBP
    

    Expression

    Bacterial

    Promoter

    BBa_J23119

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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