We narrowed to 18,715 results for: ESP

1. pMKO.1 puro GFP shRNA

   Plasmid

   #10675

   Purpose

   Negative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.

   Depositor

   William Hahn

   Article

   Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

   Insert

   GFP shRNA
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. L4440_BioBrick-hif-1-sgRNA-B

   Plasmid

   #177785

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hif-1 (hif-1 Nematode)
   

   Expression

   Bacterial

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCE27

   Plasmid

   #174405

   Purpose

   C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.

   Depositor

   Clarissa Nobile

   Article

   Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

   Insert

   NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCE35

   Plasmid

   #174409

   Purpose

   C. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.

   Depositor

   Clarissa Nobile

   Article

   Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

   Insert

   C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG_hPPP1R15B_4-713_mCherry_UK1298

   Plasmid

   #80707

   Purpose

   mammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 4-713)

   Depositor

   David Ron

   Article

   Chen et al Elife. 2015 Mar 16;4. doi: 10.7554/eLife

   Insert

   human PPP1R15B (PPP1R15B Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   CDS 4-713 no stop codon

   Promoter

   CMV

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET_RGG-GFP-RGG

   Plasmid

   #124948

   Purpose

   Expression of RGG-GFP-RGG

   Depositor

   Matthew Good, Daniel Hammer, Benjamin Schuster

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Tags

   EGFP and His

   Expression

   Bacterial

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRevTRE MKL1

   Plasmid

   #19846

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 (MRTFA Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

8. p3xFLAG-MKL1

   Plasmid

   #11978

   Purpose

   For mammalian expression of human MKL1 with a 3x FLAG tag

   Depositor

   Ron Prywes

   Article

   Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

   Insert

   MKL1 (MRTFA Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N579S T716A

   Promoter

   CMV

   Available Since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMKO.1 puro hTERT shRNA

   Plasmid

   #10688

   Depositor

   William Hahn

   Article

   Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

   Insert

   hTERT shRNA (TERT Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-tight-DDdCas9GFP-IRES-Neo

    Plasmid

    #102907

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9GFP-IRES-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA_RGG-GFP-RGG

    Plasmid

    #124930

    Purpose

    Expression of RGG-GFP-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI SMN1

    Plasmid

    #72286

    Purpose

    SMN1 exon 7 splicing cassette

    Depositor

    Elliot Androphy

    Article

    Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

    Insert

    SMN1 (SMN1 Human)
    

    Expression

    Mammalian

    Mutation

    exons 6-7-8 including introns

    Promoter

    CMV

    Available Since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pETDuet RagA-RagC

    Plasmid

    #99641

    Purpose

    Bacterial expression of wildtype Rag GTPase dimer

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAT005

    Plasmid

    #171632

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-B2M
    mNeon
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1-a and U6

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGBW-m4046887

    Plasmid

    #145730

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pET_RGG-RGG

    Plasmid

    #124941

    Purpose

    Expresion of RGG-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKDsgRNA-rssA

    Plasmid

    #89957

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    rssA gRNA
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDV24_AAV2_DART-VADAR_iRFP720-sensor

    Plasmid

    #200411

    Purpose

    AAV vector encoding a DART-VADAR sensor for iRFP720 mRNA

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    DART VADAR sensor
    

    Use

    AAV and Synthetic Biology

    Tags

    MCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-15b PAI-RBP

    Plasmid

    #45390

    Depositor

    Thomas Gelehrter

    Article

    Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

    Insert

    PAI-RBP (SERBP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-EF1a-DIO-tdTomato-WPRE

    Plasmid

    #133786

    Purpose

    Cre-dependent tdTomato fluorescent reporter for cell labeling

    Depositor

    Michael Stryker

    Article

    Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

    Insert

    pAAV-EF1a-DIO-tdTomato-WPRE
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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