We narrowed to 10,801 results for: T7

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pCMV-SpCas9-VRQR(+A61R)-P2A-EGFP (KAC1030)

Plasmid

#242665

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A61R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(A61R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + A61R mutations in SpCas9(A61R/D1135V/G1218…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+L244Q)-P2A-EGFP (LRF96)

Plasmid

#242667

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L244Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(L244Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + L244Q mutations in SpCas9(L244Q/D1135V/G12…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+N1317R)-P2A-EGFP (LRF53)

Plasmid

#242678

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N1317R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(N1317R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + N1317R mutations in SpCas9(D1135V/G1218R/N…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+A1285K)-P2A-EGFP (KAC1134)

Plasmid

#242676

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + A1285K mutations in SpCas9(D1135V/G1218R/A…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+L1245V)-P2A-EGFP (LRF49)

Plasmid

#242675

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + L1245V mutations in SpCas9(D1135V/G1218R/L…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+D1332R)-P2A-EGFP (LRF45)

Plasmid

#242681

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + D1332R mutations in SpCas9(D1135V/G1218R/D…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+L1111H)-P2A-EGFP (LRF269)

Plasmid

#242672

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + L1111H mutations in SpCas9(L1111H/D1135V/G…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+T1138K)-P2A-EGFP (LRF243)

Plasmid

#242674

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + T1138K mutations in SpCas9(D1135V/T1138K/G…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+D1332K)-P2A-EGFP (KAC966)

Plasmid

#242680

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + D1332K mutations in SpCas9(D1135V/G1218R/D…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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Skp

Plasmid

#213139

Purpose

Expresses chaperone protein SKP

Depositor

Sebastian Hiller

Article

Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

Insert

SKP (21-161) (skp E.coli)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSANG10-3F-BG4

Plasmid

#55756

Purpose

Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

Depositor

Shankar Balasubramanian

Article

Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

Insert

BG4

Tags

6xHis, pelB leader, and tri-FLAG

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET11a-4SS-HEWL

Plasmid

#39233

Depositor

Harald Schwalbe

Article

Schlorb et al J Biomol NMR. 2005 Oct;33(2):95-104.

Insert

Hen egg white lysozyme (LYZ Chicken)

Expression

Bacterial

Mutation

N-terminal Met

Promoter

T7

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ICUE3-NLS

Plasmid

#138218

Purpose

Nuclear-targeted FRET-based cAMP indicator.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

ICUE3-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET-NT*-HRV3CP

Plasmid

#162795

Purpose

Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

Depositor

Gottfried Otting

Article

Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

Insert

Human rhinovirus 14 3C protease

Tags

NT* solubility tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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sortase A pentamutant (eSrtA) in pET29

Plasmid

#75144

Purpose

evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

Depositor

David Liu

Article

Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

Insert

sortase A pentamutant

Tags

6x His

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T. Deleted amino a…

Promoter

T7

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003-mi3

Plasmid

#159995

Purpose

Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

SpyCatcher003-mi3

Tags

C-Tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pET22b_PstS_2

Plasmid

#78199

Purpose

Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

Depositor

Martin Webb

Article

Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

Insert

pstS (A17C,A197C)

Expression

Bacterial

Mutation

A17C; A197C (please see depositor comment below)

Promoter

T7

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003 S49C

Plasmid

#133448

Purpose

Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003 S49C

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Contains S49C mutation

Promoter

T7

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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SpyDock

Plasmid

#124618

Purpose

Expresses SpyDock to bind SpyTag non-covalently for affinity purification

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyDock

Tags

His6

Expression

Bacterial

Mutation

E77A mutation prevents isopeptide bond formation,…

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-6xHis-MBP-TEV site-TadA8.20

Plasmid

#194702

Purpose

Expresses N-terminal MBP fused TadA8.20 in bacteria

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

TadA8.20

Tags

6xHis and MBP

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Promoter

T7 promoter

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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