We narrowed to 9,277 results for: Pol;

1. pGGD_MiniTurboID-YFP

   Plasmid

   #222434

   Purpose

   Golden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.

   Depositor

   Dominique Bergmann

   Article

   Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

   Insert

   MiniTurboID (BirA mutant)
   

   Use

   Golden gate / green gate compatible cloning vector

   Tags

   Linker and YFP

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PX458 V3

   Plasmid

   #226957

   Purpose

   CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-UbC

   Plasmid

   #62806

   Purpose

   To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

   Depositor

   Jae Lee, Pantelis Tsoulfas

   Article

   Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

   Type

   Empty backbone

   Use

   AAV; Eucaryotic

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQFlag-USP7 WT puroR

   Plasmid

   #46751

   Purpose

   Retroviral vector that expresses wild type Flag-tagged human USP7

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CAG FLEX BARK D110A p2A mCherry

   Plasmid

   #117694

   Purpose

   CAG-FLEx-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for Cre-dependent Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs D110A peptide
   

   Use

   Cre/Lox

   Tags

   HA / FLAG

   Mutation

   D110A

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTSara

   Plasmid

   #60720

   Purpose

   Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Inserts

   N Terminal fragment of T7 RNAP split betwen 179-180
   N Terminal fragment of T7 RNAP split betwen 179-180
   

   Expression

   Bacterial

   Mutation

   T7 RNAP split between positions 179-180 and wt T7…

   Promoter

   PBAD

   Available Since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. W118-1_hTGM2_C277S-Flag

   Plasmid

   #180412

   Purpose

   lentiviral transduction of human TGM2 transcript variant 2 with a point mutation (C277S) that inactivate the enzyme and also with a C-terminal Flag tag

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   TGM2_C277S (TGM2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C277S

   Promoter

   CMV

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSP64-mHCN2

   Plasmid

   #53060

   Purpose

   Expresses alpha subunit of mouse HCN2 channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Chen et al J Biol Chem. 2000 Nov 17;275(46):36465-7

   Insert

   Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (Hcn2 Mouse)
   

   Use

   Xenopus oocyte expression

   Mutation

   E55G, R237H, and K283R polymorphisms compared to …

   Promoter

   SP6

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYES2/103Q

   Plasmid

   #1385

   Depositor

   Michael Sherman

   Article

   Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

   Insert

   huntingtin (103Q) (HTT Human)
   

   Tags

   EGFP and FLAG

   Expression

   Yeast

   Mutation

   Exon1 sequences containing the first 17 amino aci…

   Available Since

   Feb. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBactin-mNeonGreen-CI

    Plasmid

    #196850

    Purpose

    Expression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mNeonGreen
    

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLVU-coHPDL WT

    Plasmid

    #174128

    Purpose

    Expresses wild type codon-optimized HPDL in mammalian cells

    Depositor

    Michael Pacold

    Article

    Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

    Insert

    4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Codon optimized

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-CMV-mCherry-hPMCA2w/b

    Plasmid

    #111570

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2w/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Insert

    Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
    

    Tags

    mCherry

    Expression

    Bacterial

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGGB_YFP-MiniTurboID

    Plasmid

    #222433

    Purpose

    Golden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.

    Depositor

    Dominique Bergmann

    Article

    Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

    Insert

    MiniTurboID (BirA mutant)
    

    Use

    Golden gate / green gate compatible cloning vector

    Tags

    Linker and YFP

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmRFP-N1 human cofilin S3E

    Plasmid

    #50858

    Purpose

    expresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 S3E (CFL1 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    S3E psuedo-phosporylated, non-activatable

    Promoter

    CMV

    Available Since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pGM227

    Plasmid

    #220182

    Purpose

    Expresses exogenous wild-type TOMM70A

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    TOMM70A (TOMM70 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEMS2022

    Plasmid

    #49129

    Purpose

    AAV plasmid with DBH promoter (PRS2/3:Con) driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-PRS2/3:Con-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    DBH

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pCA13-pHR-SFFV-HaloTag-FBL

    Plasmid

    #199444

    Purpose

    Expression of HaloTag-FBL in mammalian cells

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    FBL (FBL Human)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCrysβ:ECFP-LexOP:mCherry-KrasG12V

    Plasmid

    #89888

    Purpose

    Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    mCherry-KRasG12V
    

    Use

    Unspecified

    Tags

    mCherry

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAP05

    Plasmid

    #186261

    Purpose

    sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

    Depositor

    Yongku Cho

    Article

    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

    Inserts

    sfGFP
    EL222 (ELI_04755 Erythrobacter litoralis)
    

    Expression

    Bacterial

    Promoter

    BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmCherry-C-p50

    Plasmid

    #180238

    Purpose

    Expresses p50 in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guo et al Proc Natl Acad Sci U S A. 2016 Sep 6;113

    Insert

    p50 (DCTN2 Human)
    

    Tags

    pmCherry-C1

    Expression

    Mammalian

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge