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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,582 results for: eng

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Showing: 5821 - 5840 of 14582 results

  3. pCDH-EF1-E-'No'Mi-Red-T2A-Puro

    Plasmid
    #246642
    Purpose
    EF-1alpha driven EV reporter E-'No'Mi-R, a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins. Nanoluc and Flag tag are removable with TEVp.
    Depositor
    Koen Breyne
    Publication
    Obuchi et al J Extracell Vesicles. 2025 Jun;14(6):e70
    Insert
    cleavable E-NoMi-Red
    Use
    Lentiviral
    Tags
    FLAG-Nanoluciferase and mCherry
    Expression
    Mammalian
    Promoter
    EF-1alpha
    Available since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    246642-plasmid-map-sequence-id-489517

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  5. pDIV301

    Plasmid
    #204969
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and ble (bleomycin) resistance marker
    Depositor
    Uffe Mortensen
    Publication
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    ble (bleomycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    204969-plasmid-map-sequence-id-454125

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  6. pCH62 (denAsCas12a-KRAB piggyBac)

    Plasmid
    #217333
    Purpose
    piggyBac expression of denAsCas12a-KRAB
    Depositor
    Luke Gilbert
    Publication
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    denAsCas12a
    Use
    CRISPR
    Tags
    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2
    Expression
    Mammalian
    Mutation
    D908A/E174R/S542R/K548R
    Promoter
    CAG
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    217333-plasmid-map-sequence-id-426481

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  13. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Publication
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

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  14. (194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

    Plasmid
    #160742
    Purpose
    Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.
    Depositor
    Christina Woo
    Publication
    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10
    Insert
    HA-nEPEA-(EAAAK)4-OGT(4,K852A)
    Tags
    HA-Tag
    Expression
    Mammalian
    Promoter
    T7/CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160742-plasmid-map-sequence-id-332924

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  16. BE3-NLS-NLS-P2A-BLAST

    Plasmid
    #135348
    Purpose
    Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3-2XNLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    WT
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135348-plasmid-map-sequence-id-267697

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  20. pCAG-mIRFP670nuc-TEV-Xph20-ETWV

    Plasmid
    #133044
    Purpose
    Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter
    Depositor
    Matthieu Sainlos
    Publication
    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Xph20-ETWV
    Tags
    NLS, TEV protease + TEV cleavage site, and miRFP6…
    Expression
    Mammalian
    Mutation
    S63K
    Promoter
    CAG
    Available since
    Nov. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    133044-plasmid-map-sequence-id-261169

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Showing: 5821 - 5840 of 14582 results