We narrowed to 14,841 results for: flag

Showing: 5841 - 5860 of 14841 results

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pcDNA3-RIPK3[S227A]-FLAG

Plasmid

#199318

Purpose

mutated phosphorylation site (MLKL interaction inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 227 to Alanine

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1-FLAG DTM

Plasmid

#185337

Purpose

Assessing domain requirements of AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

FLAG, 6xHis

Expression

Mammalian

Mutation

deletion of aa 24-45, corresponding to the hairpi…

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1-FLAG-DCUE

Plasmid

#185339

Purpose

Assessing domain requirements of AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

FLAG, 6xHis

Expression

Mammalian

Mutation

deletion of aa 293-337 corresponding to the CUE d…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK-PUM2-rABE-Flag

Plasmid

#191386

Purpose

Doxycycline inducible expression of PUM2-rABE

Depositor

Stephen Floor

Article

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

PUM2-rABE (PUM2 Synthetic, Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

tet-inducible promoter

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-Flag-NEDD4L∆WW2

Plasmid

#166708

Purpose

expresses NEDD4L with inactivating mutation in second WW domain in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L W453A

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 453 to alanine

Promoter

CMV promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-Flag-NEDD4L∆WW3

Plasmid

#166709

Purpose

expresses NEDD4L with inactivating mutation in third WW domain in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L W525A

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 525 to alanine

Promoter

CMV promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-IBU-PFAS-FLAG

Plasmid

#168226

Purpose

Expresses human PFAS-3xFLAG fusion in mammalian cells

Depositor

Noboru Mizushima

Article

Mimura et al J Biol Chem. 2021 Jan-Jun;296:100780. do

Insert

PFAS (PFAS Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT C41CA_FLAG_Mx PylRS

Plasmid

#140013

Purpose

Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET-FLAG-eSpCas9-plus

Plasmid

#126774

Purpose

Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

eSpCas9-plus

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

K848A, R1060A; amino acids 1005-1013 replaced wit…

Promoter

T7

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

Plasmid

#129530

Purpose

Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Inserts

codon-optimized AcrIIC3Nme
sgRNA_Rosa26

Use

AAV

Tags

FLAG/NLS

Promoter

U6 and cytomegalovirus-enhancer chicken β-actin p…

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA6-LRRC52-V5-Flag-His

Plasmid

#127818

Purpose

Expression of human LRRC52 in mammalian cells

Depositor

Jiusheng Yan

Article

Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

Insert

LRRC52 (LRRC52 Human)

Tags

V5, FLAG, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1-L49V

Plasmid

#125844

Purpose

express mutant BAP1-L49V

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

L49V

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-PPP2R5E siRNA-resistant

Plasmid

#120861

Purpose

expresses dox-inducible PPP2R5E resistant to siRNA pool 2 used in manuscript

Depositor

Emily Foley

Article

Lee et al J Cell Sci. 2017 May 15;130(10):1760-177

Insert

PPP2R5E (PPP2R5E Human)

Tags

FLAG

Expression

Mammalian

Mutation

silent mutation at aa 282-284 in PPP2RE to genera…

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-PGC-1alpha2

Plasmid

#45502

Depositor

Jorge Ruas, Bruce Spiegelman

Article

Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

Insert

PGC-1alpha Isoform2 (Ppargc1a Mouse)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-Flag-dTSC2 WT

Plasmid

#111807

Purpose

Insect Expression of dTSC2

Depositor

Jun Hee Lee

Article

Kim et al Int J Mol Sci. 2015 Mar 27;16(4):7015-26

Insert

dTSC2 (gig Fly)

Tags

3xFlag

Expression

Insect

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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MDH1-PGK-FLAG-TAT-86-E

Plasmid

#35978

Depositor

Susana Valente

Article

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

transactivating protein (tat Human Immunodeficiency Virus)

Use

RNAi and Retroviral

Tags

Flag

Expression

Mammalian

Mutation

From the "E" HIV clade

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-Flag CASTOR2 ACT1

Plasmid

#84493

Purpose

overexpression of CASTOR2 ACT1 domain (aa 1-171)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 ACT1 (GATSL2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

1-171 amino acid

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-Flag CASTOR1 ACT1

Plasmid

#84491

Purpose

overexpression of CASTOR1 ACT1 domain (aa 1-169)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 ACT1 (GATSL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

1-169 amino acid

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Smad4 R100T

Plasmid

#80891

Purpose

mammalian expression of Smad4 R100T

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

Smad4 (SMAD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Pancreatic cancer mutation R100T

Promoter

CMV

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-Smad4 L43S

Plasmid

#80889

Purpose

mammalian expression of Smad4 L43S

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

Smad4 (SMAD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

pancreatic cancer mutation L43S

Promoter

CMV

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only