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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,083 results for: pET

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Showing: 5881 - 5900 of 10083 results

  1. PTPRJ-bio-His

    Plasmid
    #51816
    Purpose
    Expresses full-length Receptor-type tyrosine-protein phosphatase eta precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PTPRJ (PTPRJ Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51816-plasmid-map-sequence-id-79579

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  2. EED

    Plasmid
    #25311
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EED-6 (EED Human)
    Tags
    GST
    Expression
    Bacterial
    Available since
    Oct. 25, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. ARL6

    Plasmid
    #25524
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ARL6 (ARL6 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 31, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pTCF6 HLA-A*24:02

    Plasmid
    #180445
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*24:02
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180445-plasmid-map-sequence-id-355843

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  9. pTCF48 HLA-A*11:01

    Plasmid
    #180449
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*11:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available since
    Feb. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    180449-plasmid-map-sequence-id-355113

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  11. NEDD4L

    Plasmid
    #25169
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    nedd4l.0574.0947 (NEDD4L Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Hect Domain
    Available since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  12. pBJ4

    Plasmid
    #167133
    Purpose
    A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter
    Depositor
    Akira Mine
    Publication
    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d
    Insert
    luxCDABE
    Use
    Luciferase
    Expression
    Bacterial
    Promoter
    Kanamycin promoter
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167133-plasmid-map-sequence-id-330439

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  14. pJK615

    Plasmid
    #71699
    Purpose
    Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71699-plasmid-map-sequence-id-137034

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  15. Fast-GCaMP-EF20

    Plasmid
    #52645
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Publication
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fast-GCaMP-EF20
    Expression
    Mammalian
    Mutation
    D362N/D366N mutated from GCaMP3
    Promoter
    T7
    Available since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  16. pcDNA3.1(+)-RasAR-NES

    Plasmid
    #205567
    Purpose
    Genetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.
    Depositor
    Jin Zhang
    Publication
    Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RasAR-NES (RAF1 Synthetic, Human)
    Tags
    Nuclear export signal (NES)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205567-plasmid-map-sequence-id-409398

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  18. SUV39H1

    Plasmid
    #28113
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SUV39H1 (SUV39H1 Human)
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Mutation
    contains chromo domain only (amino acid residues …
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  19. AviTag-SpyTag003-MBP

    Plasmid
    #184227
    Purpose
    Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.
    Depositor
    Mark Howarth
    Publication
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    AviTag-SpyTag003-MBP
    Tags
    AviTag, His6, SpyTag003, and Thrombin cleavage si…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184227-plasmid-map-sequence-id-361606

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  20. pJK642

    Plasmid
    #72391
    Purpose
    Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    72391-plasmid-map-sequence-id-133988

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Showing: 5881 - 5900 of 10083 results