We narrowed to 7,027 results for: CAR

Showing: 5921 - 5940 of 7027 results

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pRExT2A-NT-BmSc

Plasmid

#232205

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy-mScarlet

Depositor

Calvin Tiengwe

Publication

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mScarlet

Expression

Bacterial

Available since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL3342 PB-PEmax-puro

Plasmid

#226674

Purpose

PiggyBac cargo vector encoding PEmax and puromycin resistance.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

PEmax

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG1a ActBT2ATetP2AP - mAGH

Plasmid

#229973

Purpose

Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-Hygro

Depositor

Martin Pelosse

Publication

Pelosse et al Synthetic Biology 2025.02.13

Insert

ActBT2ATetP2AP - mAGH

Use

CRISPR

Expression

Mammalian

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG1a ActBT2AmcherryP2AP -TagBFPH

Plasmid

#229971

Purpose

Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-Hygro

Depositor

Martin Pelosse

Publication

Pelosse et al Synthetic Biology 2025.02.13

Insert

ActBT2AmcherryP2AP -TagBFPH

Use

CRISPR

Expression

Mammalian

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJB47

Plasmid

#226306

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Publication

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with C to S CsoS2 MR1-5

Expression

Bacterial

Mutation

C292S, C310S, C351S, C369S, C467S, C485S, C526S, …

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLV.5’eGFP/sgVIM

Plasmid

#170548

Purpose

Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP without homology arms

Depositor

Jacob Giehm Mikkelsen

Publication

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

sgRNA targeting 5'- end of VIM

Use

CRISPR and Lentiviral

Promoter

U6

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pXPR_023d-sgCiBIRC6-1

Plasmid

#202447

Purpose

Expression of a CRISPRi guide targeting BIRC6

Depositor

William Hahn

Publication

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pXPR_003-sgBIRC6-1

Plasmid

#202437

Purpose

Expression of a guide RNA targeting BIRC6

Depositor

William Hahn

Publication

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_023d-sgCiBIRC6-5

Plasmid

#202448

Purpose

Expression of a CRISPRi guide targeting BIRC6

Depositor

William Hahn

Publication

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003-sgBIRC6-4

Plasmid

#202438

Purpose

Expression of a guide RNA targeting BIRC6

Depositor

William Hahn

Publication

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003-sgBIRC6-5

Plasmid

#202439

Purpose

Expression of a guide RNA targeting BIRC6

Depositor

William Hahn

Publication

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003-sgUBA6

Plasmid

#202440

Purpose

Expression of a guide RNA targeting UBA6

Depositor

William Hahn

Publication

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

UBA6 gRNA (UBA6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pPINA7A2mTFP1(F)

Plasmid

#193774

Purpose

Expresses mTFP1 under the control of PINA7 synthetic promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Publication

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

mTFP1

Use

Synthetic Biology

Expression

Bacterial

Promoter

PINA7 synthetic promoter carrying binding sites f…

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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VPS13D(∆ATG2-C/PH)^EGFP

Plasmid

#194004

Purpose

Deletion of DHPH domain of VPS13D

Depositor

Pietro De Camilli

Publication

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D (VPS13D Human)

Tags

EGFP

Expression

Mammalian

Mutation

early stop codon before DHPH

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-P C-FlagHA HPV16 E7 E80A/D81A

Plasmid

#191548

Purpose

Protein expression of HPV16 E7 E80A/D81A

Depositor

Elizabeth White

Publication

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

HPV16

Use

Retroviral

Tags

FlagHA

Mutation

E80A/D81A

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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L1_NOP1:mS-N7-Ck2

Plasmid

#136126

Purpose

L1 in position 2, example of weak promoter

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpNOP1:mScarletI-N7_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-HA-PGRMC1-1-54

Plasmid

#173472

Purpose

PGRMC1 lacking the C-terminal region downstream of the transmembrane domain

Depositor

Bill Tsai

Publication

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1 (PGRMC1 Human)

Tags

HA

Expression

Mammalian

Mutation

Deleted for amino acids 55-194

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBz106_CsoS2_MR

Plasmid

#140843

Purpose

His-tagged CsoS2 Middle Region (MR), pET-14b backbone

Depositor

David Savage

Publication

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 Middle Region (MR)

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

only amino acids 257-603

Promoter

T7

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLz26_CsoS2_N1-N2_GFP

Plasmid

#140851

Purpose

His-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backbone

Depositor

David Savage

Publication

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeats 1-2 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBz110_CsoS2_CTD

Plasmid

#140844

Purpose

His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone

Depositor

David Savage

Publication

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 C-terminal domain (CTD)

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

deleted amino acids 1-594

Promoter

T7

Available since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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