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pFTK006

Plasmid

#171278

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Ptef EF1-subunit ANIA_02063 promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK005

Plasmid

#171277

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Ptef1 ANIA_04218 promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK003

Plasmid

#171275

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Patp9 An04g08190 promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK002

Plasmid

#171274

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PgndA An11g02040 promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK001

Plasmid

#171273

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Pact Pc20g11630 promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-NL2CT

Plasmid

#163623

Purpose

mouse NL2 N-terminally tagged with GST

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse NL2 (Nlgn2 Mouse)

Tags

GST

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pStA0::J23116

Plasmid

#114331

Purpose

Promoter J23116 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

Promoter J23116

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::RBSc42

Plasmid

#114338

Purpose

RBS RBSc42 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

RBS RBSc42

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::L3S2P55

Plasmid

#114339

Purpose

Terminator L3S2P55 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

Terminator L3S2P55

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pM1GG

Plasmid

#108200

Purpose

M. sedula PCNA1

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

M. sedula PCNA1

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-GFP-Ubl4A-H2(108-130)

Plasmid

#87203

Purpose

To express Ubl4A (108-130 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 108-130 are inserted.

Promoter

CMV

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-GFP-Ubl4A-H1(90-107)

Plasmid

#87202

Purpose

To express Ubl4A (90-107 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 90-107 are inserted.

Promoter

CMV

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-GFP-Ubl4A-C(90-157)

Plasmid

#86923

Purpose

To express Ubl4A (90-157 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 1-89 are deleted

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-mRuby-WDR34

Plasmid

#66599

Purpose

Expresses WDR34 as a fusion with monomeric mRuby

Depositor

David Stephens

Article

Asante et al J Cell Sci. 2014 Nov 1;127(21):4774-87.

Insert

WDR34 (WDR34 Human)

Use

Lentiviral

Tags

mRuby

Expression

Mammalian

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pET28CLY8

Plasmid

#21767

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 8 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pSP64T Activin betaB (DM#108)

Plasmid

#15045

Depositor

Douglas Melton

Article

Thomsen et al Cell. 1990 Nov 2. 63(3):485-93.

Insert

Activin Beta b (inhbb Frog)

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pB-EFS-FastFUCCI-IRES-3xnls-mTagBFP2 (JDW 1515)

Plasmid

#242600

Purpose

A PiggyBac expression vector containing the core promoter from human EF1a driving FastFUCCI to label cell cycle state followed by an IRES-3xnls-mTagBFP2.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

human EF1a

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI-IRES-myr-mTagBFP (JDW 1502)

Plasmid

#242597

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an IRES and a membrane targeted mTagBFP2

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-BLAST-CMV-LiCre-WPRE (JDW 1495)

Plasmid

#242594

Purpose

Lentiviral vector with a CMV promoter driving a light inducible cre (LiCre).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LiCre

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pME-zFUCCI-P2A-H2A-tdiRFP (JDW 1380)

Plasmid

#242559

Purpose

Gateway middle entry clone containing a zebrafish FUCCI reporter followed by H2A tdiRFP reporter; For visualizing cell cycle state.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)

Use

Gateway Cloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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