We narrowed to 59,538 results for: cloning

1. pL0M-S-mTurquoise2-EC18152

   Plasmid

   #137074

   Purpose

   Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

   Depositor

   Julian Hibberd

   Article

   Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

   Insert

   mTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAP-BAC

   Plasmid

   #120229

   Purpose

   TAR cloning in yeast and low-copy maintenance of large constructs in E. coli

   Depositor

   Bradley Moore

   Article

   Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMSCV-FLIP-puro-dsRed-GFP-miRNA

   Plasmid

   #32704

   Depositor

   Hans Clevers

   Article

   Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

   Insert

   emGFP-miRNA
   

   Use

   Cre/Lox, RNAi, and Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PB-hEF1a-MG

   Plasmid

   #175504

   Purpose

   All-in-one piggyBac transposon destination vector for hEF1a constitutive expression of Mega Gate cloned elements

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   hEF1a

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDHt-SK

   Plasmid

   #63615

   Purpose

   The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

   Depositor

   Seogchan Kang

   Article

   Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

   Type

   Empty backbone

   Use

   Fungal expression

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJRA107

   Plasmid

   #209340

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

   Plasmid

   #227774

   Purpose

   Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

   Depositor

   Bushra Raj

   Article

   Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

   Insert

   loxP-dsR-loxP
   

   Use

   CRISPR

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Bxb1-GA Donor_hEF1a

   Plasmid

   #229779

   Purpose

   Bxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment marker

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL4.23-MYC

   Plasmid

   #86461

   Purpose

   A modified version of the Promega pGL4.23 vector. Contains the MYC promoter in place of the minimal promoter and polyadenylation signals flanking the enhancer cloning site.

   Depositor

   Eric Lander

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   MYC

   Available Since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p221z-CAS9p-TagRFP-t35s

    Plasmid

    #118386

    Purpose

    Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Insert

    CAS9p-TagRFP
    

    Use

    CRISPR

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 7xSMAD_RE::GrRenilla

    Plasmid

    #178319

    Purpose

    SMAD GreenRenilla luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    SMAD GreenRenilla Reporter
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p663-UBC-miniTurbo-V5-CDKL5_IDG-K

    Plasmid

    #170578

    Purpose

    Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDKL5 (CDKL5 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    E854G- please see depositor comments

    Promoter

    Ubiquitin

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL4.23-MYC-e3

    Plasmid

    #120341

    Purpose

    pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Insert

    chr8:129056967-129058438 (hg19)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    MYC

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p5E-CMVSP6

    Plasmid

    #138356

    Purpose

    Gateway compatible 5' entry clone contains Cytomegalovirus and SP6 dual promoter(CMVSSP6)

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    CMV-SP6 promoter
    

    Use

    Gateway compatible

    Promoter

    CMV-SP6

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLCS110

    Plasmid

    #209331

    Purpose

    Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mStayGold fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL4.23-MYC-e5

    Plasmid

    #120343

    Purpose

    pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Insert

    chr8:130594100-130596043 (hg19)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    MYC

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL4.23-MYC-e4

    Plasmid

    #120342

    Purpose

    pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Insert

    chr8:129060477-129062593 (hg19)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    MYC

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pME-Gal80ts

    Plasmid

    #51619

    Purpose

    vertebrate expression of full-length temperature-sensitive Gal80 with stop

    Depositor

    Joshua Bonkowsky

    Article

    Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

    Insert

    Gal80ts (GAL80 Budding Yeast)
    

    Use

    Vertebrate expression

    Mutation

    temperature sensitive (G203V, G323R)*

    Available Since

    March 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5/FRT/TO GFP HSPA6

    Plasmid

    #19486

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 6 (HSPA6 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYSD085

    Plasmid

    #212922

    Purpose

    Encodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    649 Stalk
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge