We narrowed to 6,842 results for: GFP expression plasmids
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Plasmid#239783PurposeExpresses rat NF186 with a TAG codon at position 534 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.DepositorInsertNfasc (Nfasc Rat)
UseTagsHA-tagExpressionMammalianMutationK534TAGPromoterhNSE (obtained from Addgene plasmid #11606)Available sinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
hNSE-NF186(K571TAG)-HA
Plasmid#239784PurposeExpresses rat NF186 with a TAG codon at position 571 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.DepositorInsertNfasc (Nfasc Rat)
UseTagsHA-tagExpressionMammalianMutationK571TAGPromoterhNSE (obtained from Addgene plasmid #11606)Available sinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
hNSE-NF186(K604TAG)-HA
Plasmid#239785PurposeExpresses rat NF186 with a TAG codon at position 604 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.DepositorInsertNfasc (Nfasc Rat)
UseTagsHA-tagExpressionMammalianMutationK604TAGPromoterhNSE (obtained from Addgene plasmid #11606)Available sinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
hNSE-NF186(K680TAG)-HA
Plasmid#239786PurposeExpresses rat NF186 with a TAG codon at position 680 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.DepositorInsertNfasc (Nfasc Rat)
UseTagsHA-tagExpressionMammalianMutationK680TAGPromoterhNSE (obtained from Addgene plasmid #11606)Available sinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
hNSE-NF186(K809TAG)-HA
Plasmid#239787PurposeExpresses rat NF186 with a TAG codon at position 809 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.DepositorInsertNfasc (Nfasc Rat)
UseTagsHA-tagExpressionMammalianMutationK809TAGPromoterhNSE (obtained from Addgene plasmid #11606)Available sinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
GANES-DEVD-BNLS
Plasmid#50842PurposeExpresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.DepositorInsertddGFP A and ddGFP B
UseTagsNES sequence LALKLAGLDIGS placed after ddGFP A an…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
GoldenPiCS Kit
Plasmid Kit#1000000133PurposeGoldenPiCS: plasmid kit for engineering in P. pastoris (GoldenMOCS based). Overexpression of up to eight transcription units. 20 promoters, 10 terminators, 4 selection markers and 4 integration sitesDepositorAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-shMcu-1
Plasmid#181868PurposeExpresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoterDepositorInsertmitochondrial calcium uniporter (Mcu Mouse)
UseAAV, Adenoviral, and Mouse TargetingTagshrGFPExpressionMammalianMutationPromoterU6Available sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-shMcu-2
Plasmid#181867PurposeExpresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoterDepositorInsertmitochondrial calcium uniporter (Mcu Mouse)
UseAAV, Adenoviral, and Mouse TargetingTagshrGFPExpressionMammalianMutationPromoterU6Available sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPACE-ΔUGI (pRZ1836)
Plasmid#140243PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFPDepositorInsertSPACE-ΔUGI
UseTagsExpressionMammalianMutationV82G in TadA*, D10A in Cas9, R187W in pmCDA1PromoterCMVAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
CGBE1-VRQR (pBM1192)
Plasmid#140254PurposeCMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFPDepositorInserteUNG-BE4max(R33A)_VRQR-ΔUGI
UseTagsExpressionMammalianMutationR33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …PromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC
Plasmid#234370PurposeT-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAsDepositorInsertsNbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-13XGP41
gRNA 1-1 and gRNA 1-2
UseSynthetic BiologyTagsGB1, GP41, and sfGFPExpressionPlantMutationPromoterArabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…Available sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC
Plasmid#234372PurposeT-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAsDepositorInsertsNbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-24XGP41
gRNA 1-1 and gRNA 1-2
UseSynthetic BiologyTagsGB1, GP41, and sfGFPExpressionPlantMutationPromoterArabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…Available sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free ClLIS1 (M)
Plasmid#182484PurposeYeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and limonene synthase from Citrus limon (ClLIS1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS(M)
LS
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458M-53BP1-DN1S
Plasmid#131045PurposePlasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.DepositorInsertSpCas9-53BP1-DN1S (TP53BP1 Human)
UseCRISPRTagsGFP and SpCas9ExpressionMammalianMutationOnly expressing residues 1231-1644 of complete 53…PromoterCBHAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MYO10-HMM-Nanotrap
Plasmid#87255PurposeExpression plasmid to perform NanoSPD 2.0 assays in mammalian cells.DepositorInsertMYO10 (Myo10 Mouse)
UseTagsGFP-nanotrapExpressionMammalianMutationMyo10 is truncated to contain aa.1-941PromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-LaG16-APT1
Plasmid#234423PurposeExpress APT1 with the anti-GFP nanobody LaG16 fused at the N terminusDepositorInsertAPT1 fused to LaG16 (LYPLA1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-LaG16-APT2
Plasmid#234422PurposeExpress APT2 with the anti-GFP nanobody LaG16 fused at the N terminusDepositorInsertAPT2 fused to LaG16 (LYPLA2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-APT2-LaG16
Plasmid#234421PurposeExpress APT2 with the anti-GFP nanobody LaG16 fused at the C terminus separated by a flexible linkerDepositorInsertAPT2 fused to LaG16 (LYPLA2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-APT1-LaG16
Plasmid#234420PurposeExpress APT1 with the anti-GFP nanobody LaG16 fused at the C terminus separated by a flexible linkerDepositorInsertAPT1 fused to LaG16 (LYPLA1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only