We narrowed to 26,032 results for: Tec
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Plasmid#40306PurposeA monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).DepositorInsertpea APEX (Synthetic)
UseUnspecifiedMutationK14D, W41F, E112K (relative to wild-type)Available SinceSept. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTS2311-Tier1-spacer-PPGK-NLuc_TB
Plasmid#169552PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPPGK-NanoLuc Luciferase - mTagBFP fusion
ExpressionMammalianPromoterPPGKAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDB_118
Plasmid#216089PurposeCas9 [Sp] CRISPRa targeting CD47, positive controlDepositorInsertCD47 guide
UseCRISPR and Lentiviral; Positive controlsAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mNectarine
Plasmid#21717PurposeBacterial expression of pH-sensitive monomeric mNectarine red fluorescent proteinDepositorInsertmNectarine fluorescent proteins
TagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…Available SinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166
Plasmid#109328PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9Available SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-blueAKAR(T/A)
Plasmid#118471PurposeNegative-control mutant for blueAKAR biosensor.DepositorInsertblueAKAR(T/A)
Tags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRT498-3xOtUBD
Plasmid#190088PurposeExpresses 3 tandem OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsert3xOtUBD
Tags6xHis and MBPExpressionBacterialAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEL031
Plasmid#137900PurposeSTU2.0 SpyCas9 tRNA system for plant genome editingDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT493
Plasmid#139398PurposeBile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6)
UseSynthetic BiologyPromoterBile Acid inducible PromoterAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT494
Plasmid#139399PurposeBile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7)
UseSynthetic BiologyPromoterBile Acid inducible PromoterAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT449
Plasmid#139389PurposeBile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2)
UseSynthetic BiologyPromoterBile Acid inducible PromoterAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT455
Plasmid#139392PurposeaTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate), AmpR, ErmRDepositorInsertaTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate)
UseSynthetic BiologyPromoteraTC and BA inducible promotersAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
attB-puro-GA donor
Plasmid#182138PurposeattB-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertionDepositorInsertattB-GA-EGFP-EF1α-PuroR-T2A-TagBFP
ExpressionMammalianAvailable SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
NG-ABE8-dimer
Plasmid#138497PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSpCas9 (NG)
ExpressionMammalianAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDA_833
Plasmid#216086PurposeCas9 [Sp] knockout targeting CD47, positive controlDepositorInsertCD47 guide
UseCRISPR and Lentiviral; Positive controlsAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Sec61b-HA-mNG21-10
Plasmid#141213PurposeMembrane Tethered Split Fluorescent Reporter for ER RetragradeDepositorInsertSec61b-HA-mNG21-10
UseLentiviralTagsHAExpressionMammalianAvailable SinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKD184
Plasmid#90953PurposeExpresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutivelyDepositorInsertsThiosulfate response regulator
Superfolder GFP
mCherry
ExpressionBacterialPromoterBba_J23114, PltetO-1, and PphsAAvailable SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL0341 (pTarget)
Plasmid#200906PurposeEncodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.DepositorInsertpTarget
ExpressionMammalianAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
mApple-Annexin(A4)-12
Plasmid#54867DepositorAvailable SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGWB519
Plasmid#74861PurposeGateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).DepositorTypeEmpty backboneExpressionPlantAvailable SinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only