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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,276 results for: nes

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Showing: 5961 - 5980 of 16276 results

  4. KHK

    Plasmid
    #25532
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KHK (KHK Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pTBL210

    Plasmid
    #126451
    Purpose
    Expresses Cas9-XTEN-TdT in mammalian cells.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-XTEN-TdT (DNTT Streptococcus pyogenes, Human)
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    CMV
    Available since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    126451-plasmid-map-sequence-id-245970

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  6. pCSD_2XNLS-SpCas9WT-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

    Plasmid
    #107324
    Purpose
    Expresses SpCas9 fused to NmCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-NmCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 is fused to NmCas9
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107324-plasmid-map-sequence-id-204720

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  8. pET-dHeFSpCas9-VP64-6xHis

    Plasmid
    #92119
    Purpose
    Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, NLS, and VP64
    Expression
    Bacterial
    Mutation
    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…
    Promoter
    T7
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92119-plasmid-map-sequence-id-194893

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  9. pGCGFP-puro

    Plasmid
    #31266
    Depositor
    Andrew Pierce
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GCGFP
    Expression
    Mammalian
    Available since
    Sept. 19, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    31266-plasmid-map-sequence-id-14329

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  11. dCas9-GCN5 core

    Plasmid
    #179551
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179551-plasmid-map-sequence-id-354777

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  14. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027

    Plasmid
    #134611
    Purpose
    Mammalian expression of mouse KIF5A 378-1027
    Depositor
    Marvin Bentley
    Publication
    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KIF5A (Kif5a Mouse)
    Tags
    Halo
    Expression
    Mammalian
    Mutation
    WT with deletion of the 377 N-terminal residues.
    Promoter
    Chicken Beta actin
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    134611-plasmid-map-sequence-id-267752

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  16. eSpCas9(1.1)_No_FLAG_ATP1A1_G2

    Plasmid
    #86610
    Purpose
    Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86610-plasmid-map-sequence-id-168846

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  19. pGWB601NL3F10H

    Plasmid
    #141287
    Purpose
    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, BASTA R for plant)
    Depositor
    Andrew Millar
    Publication
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Type
    Empty backbone
    Use
    Gateway Cloning and Luciferase
    Tags
    FLAG (x3), HIS (x10), and NLUC
    Expression
    Plant
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    141287-plasmid-map-sequence-id-338006

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Showing: 5961 - 5980 of 16276 results