We narrowed to 139 results for: Tn5

1. pICH67131

   Plasmid

   #153223

   Purpose

   Level 1 position 1 containing the Nos promoter, NPTII (Kan resistance), and the Nos terminator

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Insert

   Nos promoter, NPTII, Nos terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGLU_105

   Plasmid

   #225787

   Purpose

   RS421 library variant 13181

   Depositor

   Nili Ostrov

   Insert

   13181_[Tn5]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGLU_115

   Plasmid

   #225795

   Purpose

   RS421 library variant 14067

   Depositor

   Nili Ostrov

   Insert

   14067_[Tn5]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGLU_113

   Plasmid

   #225794

   Purpose

   RS421 library variant 13453

   Depositor

   Nili Ostrov

   Insert

   13453_[Tn5]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGLU_111

   Plasmid

   #225792

   Purpose

   RS421 library variant 13463

   Depositor

   Nili Ostrov

   Insert

   13463_[Tn5]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGLU_104

   Plasmid

   #225786

   Purpose

   Non-functional control promoter, randomly-generated sequence of 165 with 50% GC

   Depositor

   Nili Ostrov

   Insert

   Dummy_[Tn5]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGLU_119

   Plasmid

   #225799

   Purpose

   RS421 library variant 14434 in pGLDV_18 (p15A)

   Depositor

   Nili Ostrov

   Insert

   14434_[Tn5]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGLU_112

   Plasmid

   #225793

   Purpose

   RS421 library variant 13926

   Depositor

   Nili Ostrov

   Insert

   13926_[Tn5]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CTV

   Plasmid

   #15912

   Depositor

   Klaus Rajewsky

   Article

   Thai et al Science. 2007 Apr 27. 316(5824):604-8.

   Insert

   CAG-STOP-eGFP-ROSA26TV
   

   Use

   Cre/Lox; /stop

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTXB1-TP5

    Plasmid

    #160088

    Purpose

    Bacterial expression of Tn5-APEX2 fusion protein with linker GSGAGA

    Depositor

    Pier Pandolfi

    Article

    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

    Insert

    Tn5 transposase/APEX2 peroxidase fusion protein
    

    Use

    Tags

    FLAG and Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTXB1-TP3

    Plasmid

    #160086

    Purpose

    Bacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKA

    Depositor

    Pier Pandolfi

    Article

    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

    Insert

    Tn5 transposase/APEX2 peroxidase fusion protein
    

    Use

    Tags

    FLAG and Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGM190

    Plasmid

    #69614

    Purpose

    inducible gene expression in Streptomyces

    Depositor

    Günther Muth

    Article

    Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

    Inserts

    aphII
    tsr
    rep
    sso
    TipA promoter
    dso
    

    Use

    Streptomyces- e. coli shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTXB1-TP4

    Plasmid

    #160087

    Purpose

    Bacterial expression of Tn5-APEX2 fusion protein with linker (GGGGS)4

    Depositor

    Pier Pandolfi

    Article

    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

    Insert

    Tn5 transposase/APEX2 peroxidase fusion protein
    

    Use

    Tags

    FLAG and Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTXB1-TP1

    Plasmid

    #160084

    Purpose

    Bacterial expression of Tn5-APEX2 fusion protein, no linker

    Depositor

    Pier Pandolfi

    Article

    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

    Insert

    Tn5 transposase/APEX2 peroxidase fusion protein
    

    Use

    Tags

    FLAG and Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTXB1-TP2

    Plasmid

    #160085

    Purpose

    Bacterial expression of Tn5-APEX2 fusion protein with linker PAPAP

    Depositor

    Pier Pandolfi

    Article

    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

    Insert

    Tn5 transposase/APEX2 peroxidase fusion protein
    

    Use

    Tags

    FLAG and Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRL1063a

    Plasmid

    #70687

    Purpose

    pRL1058 with Vibrio fischeri luxAB for sensing

    Depositor

    Peter Wolk

    Article

    Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88

    Insert

    Transposon Tn5-1063
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTn Donor

    Plasmid

    #236211

    Purpose

    Arabinose-inducible Tn5 InducTn-seq plasmid

    Depositor

    Matthew Waldor

    Article

    Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

    Insert

    None
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMODloxZeo3DAmp

    Plasmid

    #27182

    Depositor

    Yoh Wada

    Article

    Aoyama et al Nucleic Acids Res. 2005 . 33(5):e52.

    Insert

    lox-EM7p-Zeo-lox cassette flanked by Tn5 outer element
    

    Use

    In vitro transposion

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFG036 + pFG051

    Plasmid

    #137997

    Purpose

    Transposon mutagenesis plasmid and the cI repressor expression plasmid

    Depositor

    Sébastien Rodrigue

    Article

    Neil et al Commun Biol. 2020 Sep 22;3(1):523. doi:

    Insert

    Tn5 tnp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBAM1

    Plasmid

    #60487

    Purpose

    This plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genome

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al BMC Microbiol. 2011 Feb 22;11:38. doi: 1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCIFR-3

    Plasmid

    #233295

    Purpose

    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    msfGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    p14G

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET15bHis6Tnp

    Plasmid

    #79807

    Purpose

    Transform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutations

    Depositor

    Keji Zhao

    Article

    Lai et al Nat Methods. 2018 Sep;15(9):741-747. doi

    Insert

    His6Tnp
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCIFR-5

    Plasmid

    #233294

    Purpose

    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    msfGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    p14G-BCD2

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCIFR-8

    Plasmid

    #227666

    Purpose

    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Am) used for selecting for the integration can be removed in a later stage with pFNC.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    AmR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCIFR-6

    Plasmid

    #227665

    Purpose

    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Gm) used for selecting for the integration can be removed in a later stage with pFNC.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    msfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    p14G-BCD2

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCIFR-4

    Plasmid

    #227664

    Purpose

    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Sm) used for selecting for the integration can be removed in a later stage with pFNC.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    msfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    p14G-BCD2

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCIFR-2

    Plasmid

    #227663

    Purpose

    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Km) used for selecting for the integration can be removed in a later stage with pFNC.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    msfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    p14G-BCD2

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDmAct5C::Cas9-2A-Neo

    Plasmid

    #176679

    Purpose

    Expression of Drosophila codon optimized spCas9 under Drosophila Act5C promoter

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Drosophila codon optimized

    Promoter

    D. melanogaster Act5C

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAaePUb::Cas9-2A-Neo

    Plasmid

    #176680

    Purpose

    Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Drosophila codon optimized

    Promoter

    Ae. aegypti poly-ubiquitin (AAEL003888)

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMTG02-Amp-FRT

    Plasmid

    #128271

    Purpose

    Plasmid that produces the following fusion protein: TetR-LOV-RRRG(Degron)

    Depositor

    Gabor Balazsi

    Article

    Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Insert

    TetR-LOV-RRRG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMTG01-Amp-FRT

    Plasmid

    #128270

    Purpose

    Plasmid that produces the following fusion protein: TetR-LOV-TIP

    Depositor

    Gabor Balazsi

    Article

    Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Insert

    TetR-LOV-TIP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCB163

    Plasmid

    #37388

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

    Insert

    Dynactin 2 (DCTN2 Human)
    

    Use

    Retroviral

    Tags

    GFP, S-tag, and TEV

    Expression

    Mammalian

    Mutation

    A7P

    Promoter

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMTG03-AmpFRT

    Plasmid

    #128272

    Purpose

    TIP-LITer1.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP---CMV-TetOx2-GFP

    Depositor

    Gabor Balazsi

    Article

    Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Inserts

    TetR-LOV-TIP
    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMTG04-AmpFRT

    Plasmid

    #128273

    Purpose

    Deg-LITer1.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG---CMV-TetOx2-GFP

    Depositor

    Gabor Balazsi

    Article

    Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Inserts

    TetR-LOV-RRRG
    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pmCherry-C-p50

    Plasmid

    #180238

    Purpose

    Expresses p50 in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guo et al Proc Natl Acad Sci U S A. 2016 Sep 6;113

    Insert

    p50 (DCTN2 Human)
    

    Use

    Tags

    pmCherry-C1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ERMAP-CD4d3+4-bio

    Plasmid

    #73107

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding ERMAP with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    ERMAP (ERMAP Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pD649-HAsp-ERMAP-COMP5AP-AviTag-9xHis

    Plasmid

    #157075

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    ERMAP (ERMAP Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pD649-HAsp-ERMAP-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156510

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    ERMAP (ERMAP Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Cultivarium POSSUM Toolkit

    Plasmid Kit

    #1000000234

    Purpose

    The POSSUM Toolkit is a Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model microbes.

    Depositor

    Nili Ostrov

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only