We narrowed to 11,706 results for: 110

1. pRSF-T20S

   Plasmid

   #110805

   Purpose

   expresses Thermoplasma acidophilium 20S proteasome in E. Coli

   Depositor

   Yifan Cheng

   Article

   Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

   Inserts

   PsmB
   PsmA
   

   Use

   Tags

   tev-His

   Expression

   Bacterial

   Mutation

   N2D and Q2E

   Promoter

   T7 and T7 promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-P(Cry1)-DIO-intron336-dLUC

   Plasmid

   #110056

   Purpose

   Cry1 transcription luciferase reporter

   Depositor

   Eric Erquan Zhang

   Article

   Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

   Insert

   Cry1 promoter and luciferase (Cry1 Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cry1

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX458_Ruby

   Plasmid

   #110164

   Purpose

   Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA

   Depositor

   Philip Hublitz

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and Ruby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)

   Plasmid

   #110554

   Purpose

   miniTn7 delivery plasmid with araC-ParaBAD inducible promoter

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   araC-ParaBAD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   AraC-ParaBAD

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACEC3GH (Hyg)

   Plasmid

   #110084

   Purpose

   Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMEXC3H

   Plasmid

   #110080

   Purpose

   Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3C cleavage site - His10 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFLAG_OriM

   Plasmid

   #110097

   Purpose

   Analogous to pFLAG_attP, but containing oriM instead of attP, thus being a replicative vector.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFLAG_attP

   Plasmid

   #110095

   Purpose

   Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-HA-BHLHE40

   Plasmid

   #110154

   Purpose

   Expression of human BHLHE40 in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

   Insert

   BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A

    Plasmid

    #110555

    Purpose

    pFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.

    Depositor

    Martin Pule

    Article

    Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

    Insert

    Anti-RD114 heavy chain
    

    Use

    Tags

    mIgG2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1 alpha core promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. MP28445 – pFUSE2ss-aRD114_CLIg-mIgK

    Plasmid

    #110556

    Purpose

    pFUSE backbone with an IL2 signal sequence, expressing the variable light chain of anti-RD114 binder fused to murine Kappa constant light chain.

    Depositor

    Martin Pule

    Article

    Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

    Insert

    anit-RD114 light chain
    

    Use

    Tags

    mKappa

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1 alpha core promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-h53BP1 (siRNA resistant)

    Plasmid

    #110301

    Purpose

    Mammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)

    Depositor

    Chris Kok-Lung Chan

    Article

    Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

    Insert

    p53-binding protein 1 (TP53BP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    GAACGAGGA to GAGCGGGGC

    Promoter

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-Cas9-P2A-Puro

    Plasmid

    #110837

    Purpose

    Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-HA-SOX4

    Plasmid

    #110367

    Purpose

    Mammalian expression of HA-tagged SOX4

    Depositor

    Carlos Moreno

    Insert

    SOX4 (SOX4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Hy_pMT Flag-URH49 WT

    Plasmid

    #110127

    Purpose

    Expresses Flag-tagged human URH49 (DDX39A)

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    URH49 (DDX39A Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    April 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. EFS-GFP

    Plasmid

    #110834

    Purpose

    positive control for GFP expression

    Depositor

    Michael Lewis

    Article

    Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

    Insert

    elongation factor 1alpha binding sequence (EFS) at GFP promoter region
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-BLM

    Plasmid

    #110299

    Purpose

    Mammalian expression of a EGFP-tagged full length Bloom's syndrome helicase

    Depositor

    Chris Kok-Lung Chan

    Insert

    BLM (Bloom's Syndrome) helicase (BLM Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSET-6xTR-TUBE

    Plasmid

    #110313

    Purpose

    E. coli expression and purification of 6xTR-TUBE

    Depositor

    Yasushi Saeki

    Article

    Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

    Insert

    6xTR-TUBE (UBQLN1 Human)
    

    Use

    Tags

    N-terminal His6-T7 tag

    Expression

    Bacterial

    Mutation

    All Arg residues in the UBA domain are mutated to…

    Promoter

    T7

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1-blast shLuc

    Plasmid

    #110409

    Purpose

    shLuc (Target TTACGCTGAGTACTTCGA), silence LUC gene, blasticidin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    Luciferase
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (RNA PolIII)

    Available Since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQCXIX-Myc-hTIM3

    Plasmid

    #110893

    Purpose

    Expression of Myc-tagged hTIM3 at the cell surface

    Depositor

    Hyeryun Choe

    Article

    Jemielity et al PLoS Pathog. 2013 Mar;9(3):e1003232. doi

    Insert

    Hepatitis A virus Cellular Receptor 2 (HAVCR2) (HAVCR2 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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