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Plasmid#193762PurposeDoxycycline-inducible (TetOn) mCherry with SV40 NLS, control constructDepositorInsertNLS-mCherry
UseLentiviralTagsmCherryExpressionMutationSV40 NLS fused to mCherryPromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetOn-Cdt1-mCherry
Plasmid#193760PurposeDoxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag)DepositorInsertCdt1-mCherry (CDT1 Human)
UseLentiviralTagsmCherryExpressionMutationWTPromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP2_leu2d_NRT1.1
Plasmid#58332PurposeExpresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged proteinDepositorInsertNRT1.1 (NRT1.1 Mustard Weed)
UseTagsGFP-HisExpressionYeastMutationPromoterGAL1Available sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-Drosha
Plasmid#62520Purposewild-type human Drosha cDNA is subcloned into pEGFP-C1 vector between HindIII and KpnI sitesDepositorInserthuman Drosha full length (DROSHA Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-EGFP-T2A-RPL22-1xFlag
Plasmid#170323PurposeExpresses FLAG and EGFP tagged RPL22DepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-mCherry-T2A-RPL22-1xV5
Plasmid#170324PurposeExpresses V5 and mCherry tagged RPL22DepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmiR-96 cluster promoter
Plasmid#62517PurposemicroRNA 183-96-182 cluster promoter luciferase plasmidDepositorInsertpromoter of microRNA-183-96-182 cluster
UseTagsRenSP (optimized renilla luciferase gene)ExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-Drosha218-1374
Plasmid#62523PurposeN-terminal aa1-217 of Drosha is deletedDepositorInserthuman Drosha with aa 1-217 deleted (DROSHA Human)
UseTagsGFPExpressionMammalianMutationdeleted amino acids 1-217PromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-Drosha270-1374
Plasmid#62524PurposeN-terminal aa1-269 of Drosha is deletedDepositorInserthuman Drosha with aa 1-269 deleted (DROSHA Human)
UseTagsGFPExpressionMammalianMutationdeleted amino acids 1-269PromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-DroshaS300A
Plasmid#62526PurposeSerine 300 of Drosha is mutated to alanineDepositorInserthuman Drosha with serine 300 changed to alanine (DROSHA Human)
UseTagsGFPExpressionMammalianMutationchanged serine 300 to alaninePromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-DroshaS302A
Plasmid#62527PurposeSerine 302 of Drosha is mutated to alanineDepositorInserthuman Drosha with serine 302 changed to alanine (DROSHA Human)
UseTagsGFPExpressionMammalianMutationchanged serine 302 to alaninePromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-DroshaK382R
Plasmid#62519PurposeLysine 382 of Drosha was mutated to arginineDepositorInserthuman Drosha with lysine 382 changed to arginine (DROSHA Human)
UseTagsGFPExpressionMammalianMutationchanged lysine 383 to argininePromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cdt1-mCherry-Puro
Plasmid#193763PurposeDoxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertCdt1-mCherry (CDT1 Human)
UseLentiviralTagsWTExpressionMutationhuman Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cdt1ΔPIP-mCherry-Puro
Plasmid#193765PurposeDoxycyline-inducible (TetOn) human Cdt1ΔPIP (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertCdt1ΔPIP-mCherry (CDT1 Human)
UseLentiviralTagsmCherryExpressionMutationPIP degron removed (a.a.1-19 removed)PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-DIO-P-EF1a-RPL22-3xHA-IRES-YFP
Plasmid#170327PurposeDouble-floxed inverse Orientation HA and IRES-YFP tagged RPL22DepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
P-rTetO-EGFP-T2A-RPL22-3xHA
Plasmid#170326PurposeTetracycline-inducible expression of HA and EGFP tagged RPL22DepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-mCherry-hCdt1(1-100)ΔCy
Plasmid#193759PurposeExpresses N-CRL4Cdt2 reporter(mCherry-hCdt1(1-100)ΔCy)DepositorInsertmCherry-hCdt1(1-100)ΔCy (CDT1 Human)
UseLentiviralTagsExpressionMutationhuman Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…PromoterEF1aAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-DroshaS300/302A
Plasmid#62530PurposeSerines 300 and 302 of Drosha are mutated to alaninesDepositorInserthuman Drosha with serines 300 and 302 changed alanines (DROSHA Human)
UseTagsGFPExpressionMammalianMutationchanged serines 300 and 302 to alaninesPromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro
Plasmid#170317PurposeExpresses HA and EGFP tagged RPL22, puromycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro
Plasmid#170320PurposeExpresses HA and mCherry tagged RPL22, puromycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only