Addgene

We narrowed to 111,239 results for: Cel

Showing: 41 - 60 of 111239 results

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pFRT_TO_FlagHA_CELF1

Plasmid

#106099

Purpose

Inducible gene expression of FlagHA - CELF1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

CELF1 (CELF1 Human)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCellFree_G06_ZIKVC

Plasmid

#145349

Purpose

Cell-free expression vector for Zika virus Capsid protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Capsid

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCellFree_G10

Plasmid

#67144

Purpose

Gateway destination vector for cell-free expression with C-term sfGFP 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

HIS and sfGFP

Expression

Mutation

Promoter

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G08

Plasmid

#67142

Purpose

Gateway destination vector for cell-free expression with C-term mCherry myc-tag tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His-MYC and mCherry

Expression

Mutation

Promoter

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G05

Plasmid

#67139

Purpose

Gateway destination vector for cell-free expression with N-term 8xHis mCherry tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His and mCherry

Expression

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G06

Plasmid

#67140

Purpose

Gateway destination vector for cell-free expression with C-term mCherry 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

HIS and mCherry

Expression

Mutation

Promoter

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G09

Plasmid

#67143

Purpose

Gateway destination vector for cell-free expression with N-term 8xHis sfGFP tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His and sfGFP

Expression

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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dg264_pENTR_ChronosCel

Plasmid

#66102

Purpose

Entry clone containing Chronos coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

Depositor

Dominique Glauser

Article

Schild et al Genetics. 2015 May 28. pii: genetics.115

Insert

Chronos

Use

Gateway

Tags

Expression

Mutation

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_CELF2

Plasmid

#106102

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF2

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting CELF2 (CELF2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_CELF1

Plasmid

#106101

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting CELF1 (CELF1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1

Plasmid

#145369

Purpose

Gateway destination vector for cell-free and mamalian expression with N-term 8xHis and eGFP tag

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Type

Empty backbone

Use

Tags

His and eGFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_SCEL_WT

Plasmid

#81870

Purpose

Gateway Donor vector containing SCEL , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SCEL (SCEL Human)

Use

Gateway entry vector

Tags

Expression

Mutation

M124V; P201T

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SCEL_p.S10Y

Plasmid

#81522

Purpose

Gateway Donor vector containing SCEL ,part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SCEL (SCEL Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S10Y; M124V; P201T

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CYP3A4

Plasmid

#67104

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CYP3A4 (CYP3A4 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCCL-CellCycle

Plasmid

#132429

Purpose

Expressing Fucci cell cycle indicator in mammalian cells

Depositor

John M. Greally

Article

Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

Insert

hCdt, hGeminin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CYP2D6

Plasmid

#67090

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CYP2D6 (CYP2D6 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TFEC

Plasmid

#67060

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFEC (TFEC Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pLX317-CELF4

Plasmid

#115445

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

CELF4 (CELF4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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FlagETR3 (CELF2)

Plasmid

#96900

Purpose

expression of Flag-tagged human CELF2 (aka CUGBP2)

Depositor

Thomas Cooper

Article

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

human CELF2 cDNA (CELF2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 IL6

Plasmid

#67061

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IL6 (IL6 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only