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Showing: 41 - 60 of 15526 results

  3. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid
    #60224
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Mutation
    Promoter
    EFS, No promoter, and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60224-plasmid-map-sequence-id-418949

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  5. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA- Pconstitutive-sgRNA(Sth3)_blaA

    Plasmid
    #133347
    Purpose
    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets cpaA and second one targets blaA (from Caulobacter crescentus)
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_cpaA and sgRNA_blaA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133347-plasmid-map-sequence-id-269540

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  6. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaA

    Plasmid
    #133348
    Purpose
    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus)
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_blaA and sgRNA_cpaA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133348-plasmid-map-sequence-id-267409

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  7. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-enOsCas12f1-NLS_pA_AAV2 ITR

    Plasmid
    #197028
    Purpose
    AAV vector encoding a human codon-optimized enOsCas12f1 driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized enOsCas12f1
    Use
    AAV
    Tags
    Expression
    Mutation
    D52R / T132R
    Promoter
    MHCK7, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197028-plasmid-map-sequence-id-388968

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Showing: 41 - 60 of 15526 results