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We narrowed to 5,147 results for: PID

Showing: 41 - 60 of 5147 results

  6. pGG431

    Plasmid
    #165615
    Purpose
    Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165615-plasmid-map-sequence-id-330960

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  7. pGG442

    Plasmid
    #165616
    Purpose
    Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
    Use
    CRISPR
    Tags
    3x HA, SV40 NLS, Zif268, and c-Myc-like NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165616-plasmid-map-sequence-id-330958

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  8. pGG369

    Plasmid
    #165606
    Purpose
    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…
    Expression
    Bacterial
    Mutation
    Recoding of BsaI site in the AmpR cassette, two B…
    Promoter
    lacUV2 driving ω-dCas9 and UV5 driving sgRNA
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165606-plasmid-map-sequence-id-330964

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  18. pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10

    Plasmid
    #228735
    Purpose
    Expresses domain inlaid iNme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells
    Depositor
    Erik Sontheimer
    Article
    Bamidele et al bioRxiv November 07, 2024
    Insert
    Domain inlaid iNme2Smu nCas9 (D16A)-i1-L10
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228735-plasmid-map-sequence-id-453996

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  19. TFORF2897

    Plasmid
    #143656
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MTA2 (MTA2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143656-plasmid-map-sequence-id-400482

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  20. pSN840

    Plasmid
    #184832
    Purpose
    Expresses human KIF5A(∆exon27) and human KLC1 in insect cells
    Depositor
    Kyoko Chiba
    Article
    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g
    Inserts
    KIF5A (∆exon27) (KIF5A Human)
    KLC1 (KLC1 Human)
    Use
    Cre/Lox
    Tags
    His-FLAG and mScarlet-2xStrepII
    Expression
    Insect
    Mutation
    ∆exon27 form of human KIF5A
    Promoter
    p10 and polyhedrin
    Available since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    184832-plasmid-map-sequence-id-367218

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Showing: 41 - 60 of 5147 results