We narrowed to 355 results for: plasmid transfection

1. pNHT7::YB1::sNluc1

   Plasmid

   #186754

   Purpose

   An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1, CAI = 0.925) with y-box binding protein 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNHT7::ENO::sNluc1

   Plasmid

   #186755

   Purpose

   An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with enolase 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNHT7::RPL10::sNluc1

   Plasmid

   #186756

   Purpose

   An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL10 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNHT7::RPL15::sNluc1

   Plasmid

   #186757

   Purpose

   An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL15 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNHT7::YB1::sNluc0

   Plasmid

   #186758

   Purpose

   An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0, CAI = 1.0) with y-box binding protein 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNHT7::YB1::sNluc2

   Plasmid

   #186759

   Purpose

   An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2, CAI =0.713) with y-box binding protein 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNHT7::YB1::sNluc3

   Plasmid

   #186760

   Purpose

   An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc3, CAI = 0.663) with y-box binding protein 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNHT7::YB1::sNluc4

   Plasmid

   #186761

   Purpose

   An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc4, CAI = 0.743) with y-box binding protein 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNHT7::YB1::sNluc5

   Plasmid

   #186762

   Purpose

   An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc5, CAI = 0.633) with y-box binding protein 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNHT7::UMSBP::sNluc2_ORF2

    Plasmid

    #186766

    Purpose

    An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at a start codon further downstream of the endogenous gene's start codon..

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPGK-empty

    Plasmid

    #35094

    Depositor

    David Segal

    Article

    Bhakta et al Methods Mol Biol. 2010;649:3-30.

    Insert

    deletion of zinc fingers and nuclease
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    use as a transfection balance for pPGK-ZFN plasmi…

    Promoter

    PGK

    Available Since

    May 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-E-Flag

    Plasmid

    #101858

    Purpose

    Evi-1 flag tagged for transfection into mammalian cells

    Depositor

    Archibald Perkins

    Insert

    Evi-1 (Mecom Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT2iC6Dronpa2nlsER4(#636)

    Plasmid

    #184067

    Purpose

    cyclofen-inducible Dronpa2 nuclear relocation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Insert

    Dronpa2-2nls-ERT2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT2iC6FAST1nls2ER4(#638)

    Plasmid

    #184068

    Purpose

    cyclofen-inducible YFAST nuclear relocation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Insert

    YFAST-2nls-ERT2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Clim Act MCH (CAM)

    Plasmid

    #104443

    Purpose

    Expresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.

    Depositor

    Elena Casacuberta, Inaki Ruiz-Trillo

    Insert

    mCherry
    

    Use

    Expression in corallochytrium (single-celled euka…

    Promoter

    actin

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Clim EF1 MCH (CEM)

    Plasmid

    #104444

    Purpose

    Expresses mCherry under elongation factor 1 promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.

    Depositor

    Elena Casacuberta, Inaki Ruiz-Trillo

    Insert

    mCherry
    

    Use

    Expression in corallochytrium (single-celled euka…

    Promoter

    EF1

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Clim Tub MCH (CTM)

    Plasmid

    #104445

    Purpose

    Expresses mCherry under tubulin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.

    Depositor

    Elena Casacuberta, Inaki Ruiz-Trillo

    Insert

    mCherry
    

    Use

    Expression in corallochytrium (single-celled euka…

    Promoter

    tubulin promoter

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-ME-Flag

    Plasmid

    #101860

    Purpose

    Mds1-Evi-1 flag tagged for transfection into mammalian cells

    Depositor

    Archibald Perkins

    Insert

    Mds1-Evi-1 (Mecom Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCSThaP6ERT(#184)

    Plasmid

    #184103

    Purpose

    cyclofen-inducible mouse Pax6 activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Insert

    HA-MmPax6-ERT2
    

    Use

    Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6+T7

    Available Since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCSKaedenERT(#100)

    Plasmid

    #184064

    Purpose

    cyclofen-inducible Kaede nuclear relocation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Insert

    Kaede-nls-ERT2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available Since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge