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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,247 results for: Ncl

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Showing: 581 - 600 of 1247 results

  6. pET28-RPL3-1~41-mEGFP(A206K)-TwStrep

    Plasmid
    #208628
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 1~41, other residues not included
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208628-plasmid-map-sequence-id-433639

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  7. pET28-RPL3-53~77-mEGFP(A206K)-TwStrep

    Plasmid
    #208632
    Purpose
    for E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 53~77, other residues not included
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208632-plasmid-map-sequence-id-418343

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  8. pET28-RPL3-1~77-mEGFP(A206K)-TwStrep

    Plasmid
    #208624
    Purpose
    for E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 1~77, other residues not included
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208624-plasmid-map-sequence-id-418336

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  11. miniUAA5

    Plasmid
    #78484
    Purpose
    1336nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail
    Depositor
    Allan Jacobson
    Article
    Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d
    Insert
    CAN1-LUC
    Expression
    Bacterial
    Available Since
    Aug. 31, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. miniUAA6

    Plasmid
    #78485
    Purpose
    1559nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail
    Depositor
    Allan Jacobson
    Article
    Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d
    Insert
    CAN1-LUC
    Expression
    Bacterial
    Available Since
    Aug. 31, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. miniUAA4

    Plasmid
    #78483
    Purpose
    1105nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail
    Depositor
    Allan Jacobson
    Article
    Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d
    Insert
    CAN1-LUC
    Expression
    Bacterial
    Available Since
    Aug. 31, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. miniUAA2

    Plasmid
    #78481
    Purpose
    600nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail
    Depositor
    Allan Jacobson
    Article
    Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d
    Insert
    CAN1-LUC
    Expression
    Bacterial
    Available Since
    June 23, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. miniUAA3

    Plasmid
    #78482
    Purpose
    882nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail
    Depositor
    Allan Jacobson
    Article
    Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d
    Insert
    CAN1-LUC
    Expression
    Bacterial
    Available Since
    June 23, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pAG/MNase

    Plasmid
    #123461
    Purpose
    Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.
    Depositor
    Steven Henikoff
    Article
    Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1
    Insert
    Protein A-Protein G-Micrococcal Nuclease
    Tags
    6xHIS and HA
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    123461-plasmid-map-sequence-id-417805

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Showing: 581 - 600 of 1247 results