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Plasmid#128837PurposeE. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.DepositorInsertrpoZ (rpoZ E. coli)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Influenza-FPV-HA-mEGFP
Plasmid#127810PurposeExpression vector Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion protein, plasma membrane localizationDepositorInsertInfluenza A virus FPV (H7N1) hemagglutinin mEGFP fusion
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCHW110033
Plasmid#222441PurposeLevel 0, CDS module (AATG-GCTT) containing Cre recombinase.DepositorInsertCre recombinase
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW110035
Plasmid#222444PurposeLevel 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop.DepositorInsertLoxP-mCherry-stop
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbS9a-RFP
Plasmid#126172PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23113Available sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
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His-tagged FL Rec8
Plasmid#45243Purposeraise antibody against Rec8DepositorInsertRec8 (Rec8 Mouse)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCHW110031
Plasmid#222437PurposeLevel 0, promoter module (GGAG-CCAT) containing CBS4 and CMV1 promoters.DepositorInsertCBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbB19k-GFP
Plasmid#126135PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23105Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSU-araC-Cas9
Plasmid#120423PurposeMultigene editing in the Escherichia coli genome using the CRISPR-Cas9 systemDepositorInsertCas9
UseCRISPRTagsExpressionBacterialMutationPromoteraraBADAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCHW110032
Plasmid#222438PurposeLevel 0, promoter module (GGAG-AATG) containing CBS4 and CMV1 promoters.DepositorInsertCBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW00011
Plasmid#222439PurposeLevel 0, CDSns module (AATG-TTCG) containing S. cerevisiae CUP2.DepositorInsertCUP2 (Saccharomyces cerevisiae) (CUP2 Budding Yeast)
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD441_WT_hi
Plasmid#128857PurposeHigh copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AncMEcoGII291
Plasmid#205024PurposeExpressing engineered M.EcoGII with higher thermostability and DNA methylation activityDepositorInsertAncestral M.EcoGII 291
UseTagsHis6x tagExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW100002
Plasmid#222440PurposeLevel 0, CDS module (AATG-GCTT) containing rice OsL5.DepositorInsertOsL5 (Oryza sativa)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AncMEcoGII250
Plasmid#205025PurposeExpressing engineered M.EcoGII with higher thermostabilityDepositorInsertAncestral M.EcoGII 250
UseTagsHis6x tagExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pBAD.Sigma32.I54N
Plasmid#59982PurposeExpresses sigma32 I54N mutant in E. coli to render a heat-shock like cellular folding environmentDepositorInsertSigma32 I54N
UseTagsExpressionBacterialMutationI54NPromoterAvailable sinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLA230
Plasmid#11724DepositorInsertb-lactamase
UseTagsExpressionBacterialMutationGAA for Glu26 changed to TAA (premature stop codo…PromoterAvailable sinceAug. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pXY027
Plasmid#98915PurposeExpress FtsZ tagged with GFP in E coliDepositorInsertFtsZ
UseTagsGFPExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only