We narrowed to 2,424 results for: dcas9

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Donor idCas9-VP64-Blast

Plasmid

#255021

Purpose

Donor plasmid targeting the AAVS1 human locus to express dCas9-VP64 upon dox induction

Depositor

Danwei Huangfu

Publication

Pulecio et al Cell Genom. 2026 Mar 18:101189. doi: 10.

Insert

dCas9-VP64

Tags

3x FLAG

Expression

Mammalian

Promoter

Tight TRE

Available since

May 20, 2026

Availability

Academic Institutions and Nonprofits only

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Donor idCas9-KRAB-Blast

Plasmid

#255020

Purpose

Donor plasmid targeting the AAVS1 human locus to express dCas9-KRAB upon dox induction

Depositor

Danwei Huangfu

Publication

Pulecio et al Cell Genom. 2026 Mar 18:101189. doi: 10.

Insert

dCas9-KRAB

Tags

3x FLAG

Expression

Mammalian

Promoter

Tight TRE

Available since

May 20, 2026

Availability

Academic Institutions and Nonprofits only

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IF405: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-p300core

Plasmid

#121825

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-dCas9/p300core (open)

Use

Gateway Cloning and Synthetic Biology

Tags

NLS

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IA304: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-VPR

Plasmid

#121822

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-dCas9/VPR (open)

Use

Gateway Cloning and Synthetic Biology

Tags

NLS

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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Native promoter dCas9

Plasmid

#113656

Purpose

dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

Depositor

Jeffrey Barrick

Publication

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

Native promoter and dCas9

Use

CRISPR

Expression

Bacterial

Promoter

S. pyogenes Cas9 native promoter

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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p-mCherry-dCas9-PolI5M

Plasmid

#249065

Purpose

Expresses nucleus-localized dCas9 fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Publication

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

dCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pJMP8921

Plasmid

#220903

Purpose

MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PC-dCas9-novo

Depositor

Jason Peters

Publication

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJMP8932

Plasmid

#220906

Purpose

MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PG-dCas9-novo (for cloning new guides)

Depositor

Jason Peters

Publication

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDA010.188

Plasmid

#206792

Purpose

J23113.dCas9

Depositor

James Carothers

Publication

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

dCas9

Promoter

J23113

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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BPK617

Plasmid

#179297

Purpose

Expresses dCas9 fused to VP64

Depositor

Keith Joung

Publication

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

dCas9-VP64

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

CAG

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCas8

Plasmid

#82380

Purpose

dCas9 without a promoter in acsA

Depositor

Brian Pfleger

Publication

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Promoter

none

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-HDAC6.DC-FLAG

Plasmid

#30483

Depositor

Tso-Pang Yao

Publication

Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

Insert

HDAC6 (HDAC6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Catalytically inactive mutant (H216/611A)

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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p2301y-tdtomato-dCas9_DegronDead_TPLN188

Plasmid

#205409

Purpose

dCas9 guided repressor

Depositor

Jennifer Nemhauser

Publication

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

dCas9_DegronDead_TPLN188

Expression

Plant

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6M-Pak1 K299R

Plasmid

#12210

Depositor

Jonathan Chernoff

Publication

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Catalytically inactive Pak1: K299R

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pFUGW hUbC-Sga-dCas9-KRAB-T2A-GFP

Plasmid

#227009

Purpose

Lentiviral Expression of dCas9-KRAB from S. gallolyticus fused to the repressive KRAB domain

Depositor

Charles Gersbach

Publication

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Sin-dCas9-KRAB-T2A-GFP

Plasmid

#227010

Purpose

Lentiviral Expression of dCas9-KRAB from S. iniae fused to the repressive KRAB domain

Depositor

Charles Gersbach

Publication

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Spa-dCas9-KRAB-T2A-GFP

Plasmid

#227011

Purpose

Lentiviral Expression of dCas9-KRAB from S. parasanguinis fused to the repressive KRAB domain

Depositor

Charles Gersbach

Publication

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP

Plasmid

#227012

Purpose

Lentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domain

Depositor

Charles Gersbach

Publication

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCas2A

Plasmid

#82372

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, A RBS: AGGAGA

Depositor

Brian Pfleger

Publication

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Promoter

PEZ3

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLV-UbC-VP64-dCas9-VP64

Plasmid

#232179

Purpose

Lentiviral cassette that expresses 2xVP64-dCas9 fusion in mammalian cells

Depositor

Charles Gersbach

Publication

Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

Insert

VP64-dCas9-VP64

Use

Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Promoter

hUbC

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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