We narrowed to 32,671 results for: eng

1. pTS2409-Tier1-PhCMV-FURINsite

   Plasmid

   #169488

   Purpose

   Tier-1 vector encoding PhCMV-controlled FURIN protease recognition site (PhCMV-FURINsite-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven Furin cleavage site
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTS2379-Tier1-PhCMV-PEST

   Plasmid

   #169484

   Purpose

   Tier-1 vector encoding PhCMV-driven PEST tag for destabilization of proteins (PhCMV-PEST-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven PEST degradation tag
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTS2415-Tier1-PPGK-1xEAAAK

   Plasmid

   #169482

   Purpose

   Tier-1 vector encoding PPGK-controlled 1x (AEAAAK)-linker (PPGK-AEAAAK-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven EAAAK-linker
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTS2412-Tier1-PPGK-2xGS

   Plasmid

   #169481

   Purpose

   Tier-1 vector encoding PPGK-controlled 2x (GGGGS)-linker (PPGK-2xGS-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven GS-linker
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTS2378-Tier1-PhCMV-KDEL

   Plasmid

   #169479

   Purpose

   Tier-1 vector encoding PhCMV-driven ER-retention signal KDEL (PhCMV-KDEL-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven ER retention signal
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pVH8-Tier1-PhCMV-NLS

   Plasmid

   #169477

   Purpose

   Tier-1 vector encoding PhCMV-driven nuclear localization signal expression (PhCMV-NLS-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven nuclear localization signal
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPPC008.106

   Plasmid

   #171141

   Purpose

   Expression of J106 scRNA on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   J106 scRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   BBa_J23119

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS416-BXG-altTAG

   Plasmid

   #158146

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   BFP-GFP fragment (w/ TAG codon)
   

   Expression

   Yeast

   Promoter

   GAL1-10

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS416-BXG

   Plasmid

   #158145

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   BFP-GFP fragment (w/ TAG codon)
   

   Expression

   Yeast

   Promoter

   GAL1-10

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS416-BYG

    Plasmid

    #158144

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment (wild-type)
    

    Expression

    Yeast

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCTCON2-Aga1p-FAPB2.3.6

    Plasmid

    #158142

    Purpose

    Yeast display reporter for non-display yeast strains (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    FAPB2.3.6
    

    Expression

    Yeast

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEMY07AB-PRO

    Plasmid

    #127710

    Purpose

    Level 0 destination vector for a promoter with A - B scars. Clone in with BpiI

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCas2B

    Plasmid

    #82373

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGA

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Promoter

    PEZ3

    Available Since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCas8

    Plasmid

    #82380

    Purpose

    dCas9 without a promoter in acsA

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Promoter

    none

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCas7

    Plasmid

    #82379

    Purpose

    dCas9 expressed from low constituve promoter, c225, in acsA

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Promoter

    c225

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCas2E

    Plasmid

    #82376

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, E RBS: AGGGCG

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Promoter

    PEZ3

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCas2A

    Plasmid

    #82372

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, A RBS: AGGAGA

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Promoter

    PEZ3

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCas28

    Plasmid

    #82385

    Purpose

    sgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PJ23108

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCas27

    Plasmid

    #82384

    Purpose

    sgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PJ23117

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ARK2a

    Plasmid

    #81017

    Purpose

    E.coli BW25113 + JBEI-9310 (pA5c-MevT(O)-T21-MKco, JBx_039538) + JBEI-9314(pTrc99a-PMDsc, JBx_039366)

    Depositor

    Taek Soon Lee

    Article

    Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

    Inserts

    MevTo-BBa1002-pTrc-Mkco
    PMD
    

    Promoter

    LacUV5 and Trc promoter

    Available Since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge