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POM42-mCitrine

Plasmid

#69854

Purpose

N-terminal tagging with mCitrine using URA3 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCitrine minus start and stop codon

Expression

Yeast

Available Since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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LC3-mEYFP

Plasmid

#182865

Purpose

Autophagy Marker

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

rat LC3 (Anxa3 Rat)

Expression

Mammalian

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ1273

Plasmid

#44487

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, peel-1, MCS

Use

Multiple cloning site vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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mTFP1-Lysosomes-20

Plasmid

#55495

Purpose

Localization: Lysosome Membrane, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

LAMP1

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
BacPAK Flag-NFRKB

Plasmid

#19791

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

NFRKB (NFRKB Human)

Tags

Flag and His

Expression

Insect

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Syt2

Plasmid

#186688

Purpose

Expresses Synaptotagmin 2 in E. Coli

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

Synaptotagmin 2

Tags

GST and thrombin site

Expression

Bacterial

Promoter

T7

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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CT-Nano-lantern(ATP1)/pRSETB

Plasmid

#51990

Purpose

bacterial expression of a Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Tags

6xHis and chloroplast transit peptid

Expression

Bacterial

Mutation

S257G in Rluc8

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-ChrimsonR-LSSmOrange

Plasmid

#149691

Purpose

For packaging and expression of ChrimsonR-LSSmOrange in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

ChrimsonR

Use

AAV

Tags

LSSmOrange

Expression

Mammalian

Promoter

hSyn

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cas5c/pHMGWA

Plasmid

#81182

Purpose

Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas5c

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cas8c/pHMGWA

Plasmid

#81183

Purpose

Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas8c

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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