We narrowed to 8,708 results for: sgRNA

1. PlayBack-U6-Bamh1

   Plasmid

   #203298

   Purpose

   For expression of sgRNAs in a PlayBack Vector with Bamh1 as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PlayBack-U6-Bgl11

   Plasmid

   #203299

   Purpose

   For expression of sgRNAs in a PlayBack Vector with BglII as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PlayBack-U6-Not1

   Plasmid

   #203301

   Purpose

   For expression of sgRNAs in a PlayBack Vector with NotI as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PlayBack-U6-Kpn1

   Plasmid

   #203297

   Purpose

   For expression of sgRNAs in a PlayBack Vector with Kpn1 as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSUI-SynP-sfGFP

   Plasmid

   #154306

   Purpose

   Expression of sfGFP under Synthetic promoter (SynP) and cloning of the sgRNA with simple screening of positive clone by fluorescence

   Depositor

   I-Son Ng

   Article

   Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAN-PA5-RFP

   Plasmid

   #62251

   Purpose

   reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   mRFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pA5

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCut plasmid toolkit

   Plasmid Kit

   #1000000118

   Purpose

   Cas9-based kit in S. cerevisiae for optimizing heterologous gene expression. Cas9-sgRNA (pCut) plasmids for integrating into 23 loci, across all 16 yeast chromosomes.

   Depositor

   Aindrila Mukhopadhyay

   Available since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pDGE Dicot Genome Editing Kit

   Plasmid Kit

   #1000000084

   Purpose

   Golden Gate cloning-based assembly of constructs for CRISPR genome editing in dicotyledonous plants. Consists of "genome editing recipient" and "sgRNA shuttle" vectors.

   Depositor

   Johannes Stuttmann

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pSB700 lenti gRNA Blasto

   Plasmid

   #167904

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRGEB31

    Plasmid

    #51295

    Purpose

    Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plants by agrobacterium mediated transformation.

    Depositor

    Yinong Yang

    Article

    Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Rice snoRNA U3 and dual 35S promoter

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMCB320

    Plasmid

    #89359

    Purpose

    3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    puromycin resistance, mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. px459V2.0_Conc2

    Plasmid

    #134451

    Purpose

    Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPR-Cas9-ScaligD

    Plasmid

    #125688

    Purpose

    Gene knockout/in for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-mCherry

    Plasmid

    #197420

    Purpose

    Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-U6-gRNA-CBh-mCherry

    Plasmid

    #91947

    Purpose

    Expression of empty gRNA cassette and mCherry

    Depositor

    Jimok Kim

    Article

    Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLCNICK

    Plasmid

    #84653

    Purpose

    Genome editing for Lactobacillus casei Lc2W

    Depositor

    Sheng Yang

    Article

    Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

    Inserts

    Cas9 nickase
    sgRNA
    homology arms of LC2W_2179
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICSL1P1_AtU626Ter26

    Plasmid

    #191769

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. p8266 LentiCRISPR v2 hygro sgNT-1

    Plasmid

    #193977

    Purpose

    Expression of spCas9 and non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-U6

    Plasmid

    #120358

    Purpose

    piggyBac transposon vector for sgRNA expression.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSWAP_Entry_T2

    Plasmid

    #184864

    Purpose

    pSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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