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pBT13-splitC

Plasmid

#122596

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-UBF

Plasmid

#26672

Depositor

Sui Huang

Article

Chen et al J Cell Biol. 2001 Apr 2. 153(1):169-76.

Insert

UBF1 (UBTF Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only
pBBRBB-eGFP

Plasmid

#32549

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Promoter

Consitutive lac

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pSR59.4

Plasmid

#63175

Purpose

Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

OmpR (ompR )
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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HER2Nb-C AND (A OR T)-SpyTag

Plasmid

#243790

Purpose

Expresses multifunctional construct enabling the AND(OR)-gated release of a HER2 Nanobody from materials in response to HRV-3C AND (sortase eSrtA(2A9) OR TEV) treatment.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

HER2 nanobody

Tags

6x His tag and SsrA

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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EB3-mNeonGreen

Plasmid

#98881

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

EB3

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHis-hTG2

Plasmid

#100719

Purpose

Expression of human transglutaminase 2 in E.coli

Depositor

Byung Il Lee

Article

Han et al Int J Biol Macromol. 2010 Aug 1;47(2):19

Insert

TGM2 (TGM2 Human)

Tags

His

Expression

Bacterial

Mutation

silent mutation Arg580 (AGG--CGT) to enhance expr…

Promoter

T7

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-GFP-SNX9

Plasmid

#170551

Purpose

To monitor the turnover of SNX9 in lysosomes

Depositor

Christina Towers

Article

Towers et al Dev Cell. 2021 Jul 26;56(14):2029-2042.e

Insert

SNX9 (SNX9 Human)

Use

Retroviral

Tags

SNX9 in tandem with mCherry and GFP

Expression

Mammalian

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSplit-OC-U5-40K

Plasmid

#51741

Purpose

Express U5-40K-LucC fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

U5-40K (SNRNP40 Human)

Tags

FLAG and Luciferase (394-550aa)

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only
Lenti-EF1alpha-VP64-dCas9-VP64_Blast

Plasmid

#192650

Purpose

3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-Rab7(WT)

Plasmid

#112959

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Rab7

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsRoquin1_AE

Plasmid

#148637

Purpose

Mammalian Expression of HsRoquin1

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsRoquin1 (RC3H1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pOPS0359

Plasmid

#115508

Purpose

Reporter Plasmid. Taurine-inducible expression of GFP

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

sfGFP pOGG037, T-pharma pOGG003 assembled in pOGG024

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Taurine-inducible promoter of S. meliloti Promote…

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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FKBP-NTEVp(WT)

Plasmid

#137831

Purpose

Cytosolic split-TEV protease chain with FKBP Rapamycin sensing domain, N-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-NTEVp(WT)

Tags

3x FLAG and NES

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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FRB-CTEVp(WT)

Plasmid

#137832

Purpose

Cytosolic split-TEV protease chain with FRB Rapamycin sensing domain, C-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FRB-CTEVp(WT)

Tags

3x FLAG and NES

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B-NCre

Plasmid

#106368

Purpose

N-terminal part of the split-Cre System

Depositor

Johannes Hirrlinger

Article

Hirrlinger et al PLoS One. 2009;4(1):e4286. doi: 10.1371/

Insert

NCre

Use

Cre/Lox

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Cas9-NRTH

Plasmid

#136925

Purpose

Expresses Cas9-NRTH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9-NRTH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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EMTB-mNeonGreen

Plasmid

#137802

Purpose

Visualization of microtubules

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Ensconsin microtubule binding domain

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pSDM052

Plasmid

#194341

Purpose

pUC mini-Tn7 vector expressing LseCas13a

Depositor

Joseph Bondy-Denomy

Article

Mendoza et al Nature. 2020 Jan;577(7789):244-248. doi:

Insert

Cas13a(Lse)

Expression

Bacterial

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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EB3-mScarlet-I

Plasmid

#98826

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

EB3

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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